ε-聚赖氨酸盐酸盐与Nisin对蜡状芽孢杆菌的协同作用及机理  

Synergistic effect and mechanism of ε-polylysine hydrochloride and Nisin on Bacillus cereus

作　　者：史文艳 孙震[1] SHI Wen-yan;SUN Zhen(School of Food Science and Technology,Jiangnan Uniuersity,Wuxi,Jiang su 214122,China)

机构地区：[1]江南大学食品学院,江苏无锡214122

出　　处：《食品与机械》2019年第3期15-19,103,共6页Food and Machinery

摘　　要：以碱性磷酸酶、紫外吸收物、丙酮酸含量以及SDSPAGE凝胶电泳结果等为指标,通过单独及联合使用ε-聚赖氨酸盐酸盐、乳酸链球菌素(Nisin),综合评价了ε-聚赖氨酸盐酸盐、Nisin对蜡状芽孢杆菌的抑菌作用。结果表明:ε-聚赖氨酸盐酸盐与Nisin对蜡状芽孢杆菌具有协同抑制作用,Nisin主要是通过破坏细胞壁膜结构以增加细胞壁膜的通透性,而ε-聚赖氨酸盐酸盐主要是通过影响ATP酶活性、蛋白质合成与表达及能量代谢过程来影响菌体的生长代谢活动。二者联合使用,可在较低浓度下达到更好抑菌效果。The bacteriostatic action and mechanism of both against Bacillus cereus were studied by usingε-polylysine hydrochloride and nisin alone and in combination.The alkaline phosphatase content,UV absorbing content,pyruvic acid content and SDS-PAGE gel electrophoresis were used as indicators to comprehensively evaluate the antibacterial activity ofε-polylysine hydrochloride and Nisin against Bacillus cereus.The results showed thatε-polylysine hydrochloride and Nisin had a synergistic inhibitory effect on Bacillus cereus.Nisin mainly destroyed cell wall membrane structure and increased the permeability of cell wall membrane,whileε-polylysine hydrochloride mainly affected the growth and metabolism of bacteria by affecting ATPase activity,protein synthesis and expression,and energy metabolism.The combination use of theε-polylysine hydrochloride and nisin can achieve better bacteriostatic effect at lower concentrations.

关 键 词：ε-聚赖氨酸盐酸盐 乳酸链球菌素 蜡状芽孢杆菌 膜渗透性 蛋白表达 能量代谢 

分 类 号：TS202.3[轻工技术与工程—食品科学]