电针“足三里”穴对脾气虚大鼠小肠黏膜转运体SGLT1及GLUT2表达的影响  

Effect of Electroacupuncture at Zusanli on Expressions of SGLT1 and GLUT2 in Intestinal Mucosal Transporter in Rats with Deficiency of Spleen Qi

作　　者：邓婷月 曲怡[2] 董佳梓 勇入琳 王建波[2] 薛亚楠[2] 刘路 张立德[2] DENG Tingyue;QU Yi;D0NG Jiazi;Y0NG Rulin;WANG Jianbo;XUE Yanan;LIU Lu;ZHANG Lide(Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning, China;Ministry ofEducation key Laboratory of Traditional Chinese Medicine Theory and Application, Liaoning University ofTraditional Chinese Medicine, Shenyang 110847 , Liaoning, China)

机构地区：[1]辽宁中医药大学,辽宁沈阳110032 [2]辽宁中医药大学中医脏象理论及应用教育部重点实验室,辽宁沈阳110847

出　　处：《辽宁中医杂志》2019年第4期842-845,I0005,共5页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81603704);辽宁省教育厅一般项目(L20708);辽宁省自然基金项目(20170540593)

摘　　要：目的:观察电针双侧"足三里"穴对脾气虚模型大鼠小肠黏膜上皮组织内的钠依赖葡萄糖转运体(SGLT1),葡萄糖运载蛋白2(GLUT2)的基因及蛋白表达的影响。方法:将40只SD雄性大鼠随机分为正常组、脾气虚组、足三里组、非经非穴组,每组10只。所有大鼠均在SPF级动物实验中心饲养。除正常组外,其余3组均采用劳倦过度和不规则饮食复合法建立脾气虚证的大鼠模型。造模成功之后,对足三里组、非经非穴组大鼠分别进行电针"足三里"穴、非经非穴点干预处理7天。采用HE染色方法观察各组大鼠小肠黏膜组织形态变化;采用荧光定量PCR法检测大鼠小肠黏膜上皮的SGLT1和GLUT2的mRNA表达水平;采用蛋白质免疫印迹法(WB)检测大鼠小肠黏膜上皮组织内的SGLT1和GLUT2的蛋白含量变化。结果:脾气虚组大鼠小肠黏膜组织部分损伤,足三里组的小肠黏膜组织有一定程度的恢复;脾气虚组和非经非穴组大鼠小肠黏膜组织内的SGLT1和GLUT2蛋白和基因表达水平均明显低于正常组(P <0. 05);足三里组大鼠小肠黏膜组织内的SGLT1和GLUT2蛋白和基因表达水平相比于脾气虚组有所升高(P <0. 05),非经非穴组未见显著性差异(P> 0. 05)。结论:电针"足三里"穴可以调节脾气虚大鼠小肠黏膜上皮组织内的SGLT1和GLUT2基因及蛋白的异常表达,参与小肠对葡萄糖的吸收作用进而改善脾气虚证。Objective: To observe the effect of electroacupuncture at Zusanli( ST36) on the gene and protein expression of sodium dependent glucose transporter( SGLT1) and glucose carrier protein( GLUT2) in the epithelium of small intestinal mucosa of rats with spleen Qi deficiency. Methods: Forty SD rats were divided into two groups. Male rats were randomly divided into normal group,spleen Qi deficiency group,Zusanli( ST36) group,non-meridian non-acupoint group,10 rats in each group. All rats were raised in SPF level animal experimental center. The other three groups were used to establish the rat model of spleen Qi deficiency by the method of overfatigue and irregular diet. After the model was established,the rats in Zusanli( ST36) group and non-meridian non-acupoint group were treated respectively with Zusanli( ST36) group and non-meridian non-acupoint group. Electroacupuncture at Zusanli( ST36) was performed for 7 days without intervention at non-acupoint point. The morphological changes of small intestine mucosa were observed by HE staining method,and the expressions of SGLT1 and GLUT2 in rat small intestinal mucosa epithelium were detected by fluorescence quantitative PCR method. The protein contents of SGLT1 and GLUT2 in small intestinal mucosa of rats were detected by Western blot. Results: The intestinal mucosal tissue was partially damaged in spleen Qi deficiency group and recovered to some extent in Zusanli( ST36) group. The levels of SGLT1 and GLUT2 protein and gene expression in small intestinal mucosa of rats with deficiency of spleen Qi and non-acupoints were significantly lower than those of normal group( P < 0. 05),and the expression levels of SGLT1 and GLUT2 protein and gene in small intestinal mucosa of Zusanli( ST36) group were significantly lower than those of the normal group( P < 0. 05),and those of Zusanli( ST36) group were significantly lower than those of the normal group( P < 0. 05). There was no significant difference in spleen Qi deficiency group( P <0. 05),but no significant difference was found

关 键 词：足三里 SGLT1 GLUT2 

分 类 号：R-332[医药卫生]