乌骨鸡黑色素的体外抗氧化活性及其对人脐静脉内皮细胞氧化损伤的保护作用  

Protective effect of melanin from Black-bone sliky fowl on human umbilical vein endothelial cell Injury and antioxidant activity in vitro

作　　者：杨亚杰[1] 李慧[1] 胡清清 陈露露 田颖刚[1] YANG Yajie;LI Hui;HU Qingqing;CHEN Lulu;TIAN Yinggang(State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330029,China)

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330029

出　　处：《南昌大学学报（理科版）》2019年第1期59-64,共6页Journal of Nanchang University(Natural Science)

基　　金：国家自然科学基金资助项目(20962015);食品科学与技术国家重点实验室自由探索资助课题(SKLF-ZZB-201520)

摘　　要：通过测定乌骨鸡黑色素对DPPH自由基、超氧自由基和羟基自由基清除率,研究乌骨鸡黑色素的体外抗氧化能力,同时建立H_2O_2诱导的人脐静脉内皮细胞(HUVEC)细胞氧化损伤模型,MTT法测定HUVEC细胞存活率,试剂盒检测细胞乳酸脱氢酶(LDH)漏出量、丙二醛(MDA)含量和超氧化物歧化酶(SOD)活力,研究乌骨鸡黑色素对HUVEC细胞氧化损伤的保护作用。结果表明:乌骨鸡黑色素清除DPPH自由基、超氧自由基和羟基自由基的IC50值分别为0.18,0.40,2.37 mg·mL^(-1)。乌骨鸡黑色素可降低H_2O_2诱导损伤的HUVEC细胞LDH漏出量和MDA含量,提高SOD活性,并呈一定浓度依赖性。因此,乌骨鸡黑色素具有一定的体外抗氧化活性,可保护H_2O_2诱导的HUVEC细胞氧化损伤。The antioxidant capacity of black bone chicken melanin in vitro was determined by measuring the clearance rate of DPPH free radicals,superoxide radicals and hydroxyl radicals.To study the protective effect of black-bone chicken melanin on oxidative damage of HUVEC cells,the oxidative damage model of human umbilical vein endothelial cells(HUVEC) induced by H2O2 was also established.The survival rate of HUVEC cells was determined by MTT assay.The amount of lactate dehydrogenase(LDH),malondialdehyde(MDA) and superoxide dismutase(SOD) were measured by kit.The results showed that the IC50 values of DPPH free radicals,superoxide radicals and hydroxyl radicals in black bone chicken were 0.18,0.40 and 2.37 mg·mL^-1,respectively.Black bone chicken melanin can reduce the LDH leakage and MDA content of HUVEC cells induced by H2O2,and increase SOD activity in a concentration-dependent manner.Therefore,black-bone melanin has a certain ability in vitro antioxidant activity,which can protect H2O2-induced oxidative damage of HUVEC cells.

关 键 词：乌骨鸡黑色素 自由基 人脐静脉内皮细胞 氧化损伤 

分 类 号：TS253[轻工技术与工程—农产品加工及贮藏工程]