气相色谱/质谱法检测食物中毒样品中23种有机磷类农药  

Determination of 23 Organophosphorus Pesticides in Food Poisoning Samples by Gas Chromatography-mass Spectrometry

作　　者：王芹[1] 杭学宇[1] 宋鑫[1] 王露[1] 冯晓青[1] 张敏会[1] WANG Qin;HANG Xueyu;SONG Xin;WANG Lu;FENG Xiaoqing;ZHANG Minhui

机构地区：[1]淮安市疾病预防控制中心

出　　处：《环境卫生学杂志》2019年第2期172-177,183,共7页JOURNAL OF ENVIRONMENTAL HYGIENE

基　　金：淮安市科技局科技计划项目(社会发展)(HAS2015030)

摘　　要：目的建立气相色谱/质谱法检测食物中毒样品中有机磷类农药的方法。方法样品经乙腈振荡提取,QuEChERS粉末净化后离心,上清液氮吹浓缩至近干后用丙酮定容至1 mL,用气相色谱/质谱仪在选择离子扫描模式下定量检测。结果方法的线性范围为(0.1～5.0)μg/mL,线性相关系数在0.98～0.99之间,检出限(LOD)在(0.002～0.044) mg/kg,在(0.02～0.8) mg/kg添加水平下平均回收率为82.3%～124.0%,相对标准偏差(RSD)为2.5%～14.6%。结论该方法操作简便快速,能够满足食物中毒样品中有机磷的检测。Objectives To establish an analytical method for determination of organophosphorus pesticides(OPPs) in food poisoning samples by gas chromatography-mass spectrometry(GC-MS). Methods Samples were extracted by acetonitrile oscillation and centrifuged after being purified by QuEChERS powder. The supernatants were blown by nitrogen and concentrated to near dryness with acetone to a volume of 1 mL.Then the concentrated solutions were quantitatively detected by gas chromatography-mass spectrometer in selected ion scan mode. Results The method showed a linearity range of(0.1~5.0)μg/L with linear correlation coefficients of 0.98~0.99, and the Limit of Detection(LOD) was(0.002~0.044) mg/kg. The average recoveries were 82.3%~124.0% at the addition level of(0.02~0.8) mg/kg with the relative standard deviation(RSD) of 2.5%~14.6%. Conclusions This method is simple and fast and can satisfy the detection of organophosphorus in food poisoning samples.

关 键 词：气相色谱/质谱法 食物 中毒样品 有机磷 

分 类 号：R15[医药卫生—营养与食品卫生学]