双核钌配合物的DNA键合及光断裂性能  

DNA binding and photocleavage properties of a dinuclear ruthenium complex

作　　者：王爽 徐雪雪 米雅萱 郑泽宝[2] 赵晓珑[1] WANG Shuang;XU Xuexue;MI Yaxuan;ZHENG Zebao;ZHAO Xiaolong(College of Chemistry and Environmental Science,Hebei University,Baoding 071002,China;College of Chemistry, Taishan University, Taian 271021,China)

机构地区：[1]河北大学化学与环境科学学院,河北保定071002 [2]泰山学院化学系,山东泰安271021

出　　处：《河北大学学报（自然科学版）》2019年第3期254-263,共10页Journal of Hebei University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(21401038);河北省自然科学基金资助项目(B2016201122);河北省高等学校科学研究计划项目(QN2014123);保定市科学技术研究与发展计划项目(14ZF009);泰安市科学技术研究与发展计划项目(2015GX2048)

摘　　要：合成了1个新型的双核钌配合物,并通过紫外-可见吸收光谱和荧光光谱滴定、热熔链、盐效应、黏度和凝胶电泳实验研究了其与小牛胸腺DNA (ct-DNA)之间的相互作用.结果表明,由于主配体的位阻作用,该配合物通过部分插入模式与DNA键合,键合常数大于10~6 L/mol;在365 nm紫外光照射下,该配合物能够断裂pBR322 DNA,是有效的DNA断裂试剂.A dinuclear ruthenium complex has been synthesized and characterized. The calf thymus DNA (ct-DNA) binding properties of the complex are investigated by means of DNA viscosity and optical spectroscopic techniques of UV-visible absorption and emission spectral titrations, DNA thermal denaturation and reverse, salt effect and agarose gel electrophoresis. The results suggest that the complex binds to DNA through partial-intercalation mode with a large DNA binding constant of 10 6 L/mol order of magnitude. The complex is also demonstrated to be an efficient cleaver of pBR 322 DNA under irradiation at 360 nm.

关 键 词：钌配合物 DNA 键合 电泳 

分 类 号：Q523[生物学—生物化学]