荔枝多糖的超声-微波提取工艺优化及其免疫调节作用  

Optimization of Ultrasonic-microwave Assisted Extraction and Immunomodulating Activities of Polysaccharide from Litchi Fruits

作　　者：刘洋[1,2] 黄菲[2] 李巍巍[1,2] 唐小俊[2] 张瑞芬[2] 张名位[2] Liu Yang;Huang Fei;Li Weiwei;Tang Xiaojun;Zhang Ruifen;Zhang Mingwei(Department of Food Science and Technology,Huazhong Agricultural Universityy Wuhan 430070;Sericultural & Agri-Food,Research Institute Guangdong Academy of Agricultural Sciences,Key Laboratory of Functional Foods,Ministry of Agriculture and Rural Affairs,Guangdong Key Lai) oratory of Agricultural Products Processing,Guangzhou 510610)

机构地区：[1]华中农业大学食品科技学院,武汉430070 [2]广东省农业科学院蚕业与农产品加工研究所农业农村部功能食品重点实验室广东省农产品加工重点实验室,广州510610

出　　处：《中国食品学报》2019年第4期184-190,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31801498);广东省省级科技计划项目(2017A030313163;2017A070702007);广州市科学研究计划-重点项目(201804020064);广东省农业科学院院长基金项目(201713)

摘　　要：采用响应面分析法优化荔枝多糖超声-微波提取的最佳工艺条件;分析小鼠器官指数、血清溶血素、NK细胞活性、脾淋巴细胞增殖,探究荔枝多糖体内免疫调节活性。结果表明,荔枝多糖超声-微波的最佳提取工艺条件为p H 8.2,微波功率559 W,提取时间10.8 min,水/料比22.7∶1,在此提取条件下,多糖提取产率可达24.02%,高于传统的热水法、微波法和超声法。体内免疫调节活性评价实验表明:在50～200 mg/kg范围,荔枝多糖均能提高小鼠的血清溶血素水平、胸腺器官指数、NK细胞杀伤活性以及促进脾淋巴细胞增殖,且中剂量(100 mg/kg)荔枝多糖的免疫活性最强。由此表明,采用超声-微波法能显著提高荔枝多糖的提取率,且获得的荔枝多糖具有显著的体内免疫调节作用。Response surface analysis experiment was carried out to optimize the polysaccharides extraction from litchi pulp.The immunomodulatory effects of obtained litchi polysaccharides (LP) were further evaluated in normal KM mice.The results showed that the optimal extraction conditions were as follows :pH 8.2, power 559 W, extraction time 10.8 min, water/solid ratio 22.7 :1 and the yield was 24.02% under this condition, which was significantly higher than those of hot extraction, ultrasonic -assisted extraction and microwave -assisted extraction.In addition, the administration of LP (5 0 ,1 0 0 mg/kg and 200 mg/kg) remarkably enhanced serum hemolysin production, increased the thymus index, NK cells cytotoxicity and splenocyte proliferation in KM mice.Middle dose group (100 mg/kg) LP exhibited the best immunomodulatory activities among three doses.In summary, the ultrasonic-microwave assisted extraction significantly increased the yield of litchi polysaccharide and obtained litchi polysaccharides exerted their immunomodulatory activities in vivo.

关 键 词：荔枝多糖 超声微波 提取 免疫调节 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]