混菌发酵石斛酵素及其抗氧化活性研究  

Mixed Fermentation of Dendrobium Enzyme and its Antioxidant Activity

作　　者：舒旭晨 杜万根 姜东 高越 魏明 赵世光 SHU Xuchen;DU Wangen;JIANG Dong;GAO Yue;WEI Ming;ZHAO Shiguang(College of Biology and Chemistry Engineering,Anhui Polytechnic University,Wuhu 241000,China)

机构地区：[1]安徽工程大学生物与化学工程学院

出　　处：《徐州工程学院学报（自然科学版）》2019年第2期63-70,共8页Journal of Xuzhou Institute of Technology(Natural Sciences Edition)

基　　金：安徽省高校自然科学研究项目(KJ2017A101);安徽省大学生创新创业训练计划项目(201610363212,201710363199,201810363202);安徽工程大学宣城产业研究项目(B2018-03)

摘　　要：以铁皮石斛鲜条为原料,以酵母菌、乳酸菌为混菌发酵剂,对石斛酵素发酵条件进行优化研究.以单因素实验法分别考察碳源、菌种配比、温度、料液比、接种量等因素对石斛酵素发酵的影响.获取较优因素后,以羟基自由基清除率为响应值,采用数理统计学方法对蔗糖质量分数、菌种配比、发酵温度三因素进行优化.结果表明:在蔗糖质量分数8.12%、菌种配比1∶2(酵母菌:乳酸菌)、发酵温度31℃条件下,制备的石斛酵素羟基自由基清除率达到34.79%,较优化前提升了108.45%.The Dendrobium officinale enzyme is fermented by mixed cultivation of yeast and lactic acid bacteria.Single factor experiment was used to study the effect of carbon source,strain ratio,temperature,solid-liquid ratio and inoculation amount on the fermentation of Dendrobium officinale enzyme.Based on the results of single factor experiment,the sucrose content,strain ratio and fermentation temperature were optimized by mathematical statistics when targeting on hydroxyl radical scavenging rate.The results showed that the optimum conditions were 8.12% of sucrose addition,1∶2 of strain ratio and 31 ℃ of fermentation temperature,under which the hydroxyl radical scavenging rate of Dendrobium officinale enzyme was up to 34.79% and increase by 108.45%.

关 键 词：石斛酵素 抗氧化活性 响应面法 条件优化 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]