基于美拉德反应产物作为荧光和共振瑞利散射的传感平台快速检测阿斯巴甜  

A Novel Sensing Platform of Fluorescence and Resonance Rayleigh Scattering for Fast Detection of Aspartame Based on Maillard Reaction Product

作　　者：赵艳梅 张小林 周尚[3] 杨季冬 高佩[5] ZHAO Yanmei;ZHANG Xiaolin;ZHOU Shang;YANG Jidong;GAO Pei(School of Environment and Chemical Engineering,Chongqing Three Gorges University,Chongqing,404100,China;Department of Chemistry,Eastern Kentucky University,Richmond,KY 40475,United States;School of Chemistry and Chemical Engineering,Yangtze Normal University,Chongqing 408100,China;School of Chemistry and Chemical Engineering,Southwest University,Chongqing 400715,China;Department of Mathematics,Sciences and Technology,Paine College,Augusta,GA 30901,United Sates)

机构地区：[1]重庆三峡学院环境与化学工程学院,重庆404100 [2]东肯塔基大学化学系,美国肯塔基州里士满40475 [3]长江师范学院化学化工学院,重庆408100 [4]西南大学化学化工学院,重庆400715 [5]佐治亚州佩恩学院数学、科学与技术系,美国佐治亚州奥古斯塔30901

出　　处：《三峡生态环境监测》2019年第2期64-80,共17页Ecology and Environmental Monitoring of Three Gorges

基　　金：国家自然科学基金(21175015,21475014)

摘　　要：源于葡萄糖和L-精氨酸(glucose and L-Arginine,GLA)的美拉德反应的产物与Cu^2+的螯合作用形成具有良好的光学活性和水溶性的GLA-Cu^2+复合物,建立荧光(fluorescence,FL)和共振瑞利散射(resonance rayleigh scattering,RRS)的新型传感平台,可用来检测阿斯巴甜(Aspartame,APM)。研究表明,由于GLA与Cu^2+螯合,GLA的蓝色荧光将被Cu^2+淬灭,同时出现了一个增强的RRS特征峰。当加入一定量的APM后,系统的荧光恢复,RRS峰强度降低。因此,GLA-Cu^2+-APM体系形成荧光“关-开”模式和RRS“开-关”模式的传感平台,这个传感平台展现出很高的灵敏度和良好的选择性,可用于样品溶液中痕量APM的快速检测。其线性范围分别为0.3~300.0μmol/L(FL)和0.4~800.0μmol/L(RRS),检测限(LOD)为26.0 nmol/L(FL)和39.0 nmol/L(RRS)。应用此传感平台的FL法测定水样中的APM取得了令人满意的结果。A novel sensing platform with optical activity and water solubility was constructed through the direct combination of Cu^2+ with the Maillard reaction product of glucose and L-Arginine (GLA). The GLA-Cu^2+ complex was selected as sensing platform of fluorescence(FL) and resonance Rayleigh scattering(RRS) for detection of aspartame(APM). This paper demonstrates that the blue fluorescence of GLA is quenched by Cu^2+ owing to the chelating ability of GLA toward Cu^2+, but a new enhanced RRS characteristic peak appears because of the presence of Cu ion. However, the fluorescence of the system recovered and RRS intensity of the new peak decreased after the following addition of certain amount of APM. Thus, GLA-Cu^2+-APM system can act as sensing platform with switches of fluorescence turn-off-on and RRS turn-on-off for the determination of APM in sample solutions. This sensing platform exhibits excellent selectivity and sensitivity in a wide linear range of 0.3~300 μmol/L (FL) and 0.4~800 μmol/L (RRS), and limit of detection (LOD) of 26 nmol/L (FL) and 39 nmol/L (RRS), respectively, for APM tracer concentration. The applications of this sensing platform with FL method to determination of APM in water samples have demonstrated very satisfactory results.

关 键 词：美拉德反应 荧光探针 共振瑞利散射探针 阿斯巴甜 

分 类 号：O657[理学—分析化学]