UPLC/MS/MS法定性定量测定中药材中3类27种合成色素  

Qualitative and Quantitative Determination of 27 Synthetic Dyes of 3 Species in Herbs by UPLC/MS/MS

作　　者：张甦[1] 胡青[1] 孙健[1] 冯睿[1] 于泓[1] 季申[1] Zhang Su;Hu Qing;Sun Jian;Feng Rui;Yu Hong;Ji Shen(Shanghai Institute for Food and Drug Control,Shanghai 201203,China)

机构地区：[1]上海市食品药品检验所,上海201203

出　　处：《世界中医药》2019年第4期822-827,832,共7页World Chinese Medicine

基　　金：中国药典2015年版标准提高方法研究项目(中药中常用色素检测方法的研究);上海市科委技术服务平台(18DZ2292200)

摘　　要：目的:采用液相-固相萃取-超高效液相色谱-电喷雾串联质谱联用技术对27种色素建立了准确、灵敏的分析方法。方法:针对脂溶性、酸性和碱性3类色素分别采用不同的制备方法、色谱条件和电喷雾极性,采用4种不同药用部位的代表性药材进行了方法学考察。结果:表明方法线性关系良好(r≥0. 997);脂溶性和碱性色素的检测限为0. 03～3. 87 ng/g,酸性色素为0. 003～1. 94μg/g;准确度和精密度分别为70%～105%和0. 9%～6. 8%;应用于25个品种80批样品的筛查。结论:所建方法可靠、高效、准确,适用于中药材复杂基质中不同种类色素的测定。Objective: To develop a sensitive and accurate method combining liquid and solid phase extraction with ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry to analyze 27 dyes. Methods: Different preparations,chromatography conditions and polarity of ESI were applied for 3 categories of lipophilic,acidic and basic dyes respectively.Four species of herbs representing different parts of plant were selected for methodology observation. Results: The linear correlation coefficient was good(r ≥ 0.997).The LODs of oil soluble and basic dyes were 0.03~3.87 ng/g and acid dyes were 0.003~ 1.94 μg/g respectively.The accuracies and precisions were 70%~105% and 0.9%~6.8% respectively. Conclusion: After detecting 80 samples of 25 species herbs,this method proved to be reliable,efficient and accurate in the detection of different kinds of dyes in the complex matrix of herbs.

关 键 词：27种 合成色素 脂溶性 酸性 碱性 定性定量 中药材 UPLC/MS/MS 

分 类 号：R284.1[医药卫生—中药学]