响应面法优化野生五叶草莓总黄酮提取工艺  

Optimization of Extraction Process for Total Flavonoids From Five-leaf Strawberry by Response Surface Method

作　　者：孙娜[1] 王华[1] 朱秀娟[1] 胡文斌[1] 宫峥嵘 SUN Na;WANG Hua;ZHU Xiujuan;HU Wenbin;GONG Zhengrong(Department of Agriculture and Forestry Technology, Longnan Teachers College, Chengxian 742500, China)

机构地区：[1]陇南师范高等专科学校农林技术学院

出　　处：《四川理工学院学报（自然科学版）》2019年第3期1-6,共6页Journal of Sichuan University of Science & Engineering(Natural Science Edition)

基　　金：陇南师范高等专科学校教学改革项目(JXGG201720)

摘　　要：采用超声波辅助乙醇浸提法探索甘肃陇南产野生五叶草莓中总黄酮的最佳提取工艺条件。在单因素试验基础上,以料液比、超声时间、超声温度和乙醇体积分数为自变量,总黄酮提取率为因变量,利用Box-Behnken四因素三水平中心组合设计,对总黄酮提取工艺进行优化。结果表明,最佳提取工艺为:液料比12∶1(m L/g)、超声时间27 min、超声温度59℃和乙醇体积分数61%,野生五叶草莓总黄酮提取率实际可达10. 68 mg/g。超声波辅助乙醇浸提法的总黄酮得率为传统水浴回流法的2. 1倍,且该方法操作简单,重复性好。研究结果能为五叶草莓总黄酮的开发利用提供参考。The optimum extraction conditions of total flavonoids from five-leaf strawberry in Longnan City,Gansu Province are explored by ultrasonic assisted ethanol extraction. On the basis of single-factor test,taking the ratio of material to liquid,ultrasonic time,ultrasonic temperature and ethanol volume fraction as independent variables,and the extraction rate of total flavonoids as dependent variable,the extraction process of total flavonoids is optimized by using Box-Behnken four-factor three-level central combination design. The results show that the optimum extraction process is that the ratio of material to liquid is 12∶ 1( m L/g),the ultrasonic time is 27 min,the ultrasonic temperature is 59 ℃,and the volume fraction of ethanol is 61% and the total flavonoids extraction rate of the wild five-leaf strawberry can reach 10. 68 mg/g. The total flavonoid yield of ultrasonic assisted ethanol extraction is 2. 1 times higher than that of traditional water bath reflux method,and the method is simple to operate and has good repeatability. The results can be used as a reference for the development and utilization of total flavonoids in five-leaf strawberry.

关 键 词：五叶草莓 总黄酮 乙醇浸提 超声波辅助 工艺优化 响应面法 

分 类 号：TQ461[化学工程—制药化工]