葡甘露聚糖-虾青素复合物的表征及对AFB1致肉鸡肝损伤的影响  

Characterization of glucomannan-astaxanthin complex and its effect on broiler liver injury induced by AFB1

作　　者：汪文俊[1] 王胜军[1] 余诚 余程[1] 向福[3] WANG Wenjun;WANG Shengjun;YU Cheng;YU Cheng;XIANG Fu(Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China,College of Life Sciences,South-Central University for Nationalities,Wuhan 430074,China;Class 9,Grade 2,Hubei Wuchang Eperimental High School,Wuhan 430060,China;Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains,Huanggang Normal University,Huanggang 438000,China)

机构地区：[1]武陵山区特色资源植物种质保护与利用湖北省重点实验室,中南民族大学生命科学学院,武汉430074 [2]湖北省武昌实验中学高二(9)班,武汉430060 [3]大别山特色资源开发湖北省协同创新中心,黄冈师范学院,黄冈438000

出　　处：《中南民族大学学报（自然科学版）》2019年第2期184-188,共5页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：湖北省自然科学基金资助项目(2016CFB487);中央高校基本科研业务费专项资金重点资助项目(CZZ16003)

摘　　要：从红法夫酵母提取了葡甘露聚糖、虾青素并制备了葡甘露聚糖-虾青素(GM-ASTA)复合物,对其进行了红外光谱、热失重(TGA)和分子对接模拟分析,探讨了GM-ASTA复合物对黄曲霉毒素B1(AFB1)致肉仔鸡肝损伤的影响.结果表明:葡甘露聚糖和虾青素形成了复合物,前者主链通过范德华力和静电力与后者紧密结合.GM-ASTA复合物能显著地改善AFB1导致的肉鸡肝损伤后果,具有吸附AFB1并解除其毒性的双重功效.Glucomannan(GM)and astaxanthin(ASTA)were extracted from Phaffia rhodozyma and their GM-ASTA complex were prepared and investigated by infrared(IR)spectroscopy,thermogravimetric analysis(TGA)and molecular docking simulation analysis.The effects of ASTA complex on aflatoxin B1(AFB1)-induced liver injury in broilers were also discussed.The results indicated that a complex was formed by GM and ASTA,which was combined by van der Waals and electrostatic forces between the GM main chain and ASTA.GM-ASTA complex could significantly improve the liver injury induced by AFB1 in broilers and had the dual effects of absorbing and removing AFB1.

关 键 词：葡甘露聚糖 虾青素 红法夫酵母 肝损伤 黄曲霉毒素B1 肉鸡 

分 类 号：S831.5[农业科学—畜牧学]