mRNA expression of CYP4 in marine polychaete Marphysa sanguinea in response to benzo[a]pyrene  

作　　者：Wanjuan Li Huan Zhao Fuyang Ba Shaojuan Li Xiupeng Sun Dazuo Yang Yibing Zhou 

机构地区：[1]Key Laboratory of Marine Bioresource Restoration and Habitat Reparation in Liaoning Province,Dalian Ocean University,Dalian 116023,China [2]Marine and Fisheries Technology Center of Pan Shan Country,Panjin 124000,China

出　　处：《Acta Oceanologica Sinica》2019年第6期46-53,共8页海洋学报（英文版）

基　　金：The Dalian Science Fund for Distinguished Young Scholars under contract No.2016RJ06;the Natural Science Foundation of Liaoning Province under contract No.201602107;the Superior College Science Technology Research Project of Liaoning Province under contract No.ZL201701;the National Natural Science Foundation of China under contract No.41306138

摘　　要：Rapid amplification of cDNA ends (RACE) and real-time polymerase chain reaction (RT-PCR) were carried out to analyze the CYP4 gene expression in polychaete Marphysa sanguinea exposed to benzo[a]pyrene (BaP) in this study. The full length of MsCYP4 cDNA was 2 470 bp, and it encoded 512 amino acids. The deduced amino acid sequence showed 47% identity with CYP4F from frog Xenopus tropicalis and shared high homology with other known CYP4 sequences. To analyse the role of CYP4 in protecting M. sanguinea from BaP exposure, three BaP groups were established: 0.5, 5 and 50 μg/L. Polychaetes were sampled after 3, 7 and 12 d. At 0.5 μg/L, the effect of BaP on MsCYP4 gene expression increased with time prolonged. MsCYP4 gene expression curve showed Ushaped trend with time in 5 and 50 μg/L BaP groups. Therefore, MsCYP4 gene may play an important role in maintaining the balance of cellular metabolism and protecting M. sanguinea from BaP toxicity.Rapid amplification of cDNA ends(RACE) and real-time polymerase chain reaction(RT-PCR) were carried out to analyze the CYP4 gene expression in polychaete Marphysa sanguinea exposed to benzo[a]pyrene(BaP) in this study. The full length of MsCYP4 cDNA was 2 470 bp, and it encoded 512 amino acids. The deduced amino acid sequence showed 47% identity with CYP4 F from frog Xenopus tropicalis and shared high homology with other known CYP4 sequences. To analyse the role of CYP4 in protecting M. sanguinea from BaP exposure, three BaP groups were established: 0.5, 5 and 50 μg/L. Polychaetes were sampled after 3, 7 and 12 d. At 0.5 μg/L, the effect of BaP on MsCYP4 gene expression increased with time prolonged. MsCYP4 gene expression curve showed Ushaped trend with time in 5 and 50 μg/L BaP groups. Therefore, MsCYP4 gene may play an important role in maintaining the balance of cellular metabolism and protecting M. sanguinea from BaP toxicity.

关 键 词：Marphysa sanguinea CYP4 BENZO[A]PYRENE TOXICITY EFFECT 

分 类 号：P[天文地球]