Effects of Nicotine, a Plant Secondary Metabolite, on Protective Enzyme and Detoxification Enzyme of Frankliniella occidentalis  

作　　者：Liu Jiani Su Yuan Wei Wei Zhong Yu Chen Zebin Yu Lei 

机构地区：[1]School of Agriculture, Kunming University/Urban Modern Agriculture Engineering Research Center

出　　处：《Plant Diseases and Pests》2019年第2期7-10,共4页植物病虫害研究（英文版）

基　　金：Supported by University Joint General Program of Yunnan Department of Science and Technology(2017FH001-35)

摘　　要：[Objective] The paper was to explore the effects of plant secondary metabolite on protective enzymes and detoxification enzymes of quarantine pest Frankliniella occidentalis. [Method] F. occidentalis were continuously exposed to artificial diets containing nicotine from the second to fifth instars for five generations, and the effects of nicotine on protective enzymes and detoxification enzymes of larvae were determined. [Result]The activities of carboxylate esterase(CarE) and glutathione-S-transferase(GST) in midgut of the fifth instar larvae were significantly increased, and reached the maximum at the third generation(1.86 times and 1.59 times, respectively). Nicotine inhibited the activities of superoxide dismutase(SOD) and catalase(CAT). With the increase of feeding generations, the inhibitory effect of nicotine weakened. Nicotine significantly increased the activities of peroxidase(POD). [Conclusion] F. occidentalis will gradually adapt to the harm of plant toxins by starting its own detoxification enzyme system and regulating protective enzyme system after feeding on plant secondary substance nicotine.[Objective] The paper was to explore the effects of plant secondary metabolite on protective enzymes and detoxification enzymes of quarantine pest Frankliniella occidentalis. [Method] F. occidentalis were continuously exposed to artificial diets containing nicotine from the second to fifth instars for five generations, and the effects of nicotine on protective enzymes and detoxification enzymes of larvae were determined. [Result]The activities of carboxylate esterase(CarE) and glutathione-S-transferase(GST) in midgut of the fifth instar larvae were significantly increased, and reached the maximum at the third generation(1.86 times and 1.59 times, respectively). Nicotine inhibited the activities of superoxide dismutase(SOD) and catalase(CAT). With the increase of feeding generations, the inhibitory effect of nicotine weakened. Nicotine significantly increased the activities of peroxidase(POD). [Conclusion] F. occidentalis will gradually adapt to the harm of plant toxins by starting its own detoxification enzyme system and regulating protective enzyme system after feeding on plant secondary substance nicotine.

关 键 词：NICOTINE FRANKLINIELLA OCCIDENTALIS DETOXIFICATION ENZYME Protective ENZYME 

分 类 号：S[农业科学]