槟榔提取物对α-葡萄糖苷酶的抑制作用研究  

Inhibitory Effects of Areca Nut Extract on the Activity of α-Glucosidase

作　　者：宋菲[1] 张玉锋[1] 郭玉如 李瑞[1] 陈华[1] 唐敏敏[1] SONG Fei;ZHANG Yu-feng;GUO Yu-ru;LI Rui;CHEN Hua;TANG Min-min(Coconut Research Institute of Chinese Academy of Tropical Agricultural Sciences,Wenchang 571339,Hainan,China)

机构地区：[1]中国热带农业科学院椰子研究所

出　　处：《食品研究与开发》2019年第13期78-83,共6页Food Research and Development

基　　金：中国热带农业科学院基本科研业务费(1630152017017)

摘　　要：研究槟榔提取物对α-葡萄糖苷酶的抑制作用,并采用Lineweaver-Burk双倒数法分析其动力学性质。结果表明,槟榔壳、槟榔籽和槟榔花提取物对α-葡萄糖苷酶活性均具有一定的抑制作用,其半数抑制浓度IC50分别为(2.87±0.48)mg/mL、(1.50±0.31)μg/mL和(4.00±0.53)mg/mL,其中槟榔籽提取物的抑制作用明显高于阳性对照阿卡波糖[IC50为(0.71±0.09)mg/mL]。对提取物中多酚、多糖等成分进行分析,发现槟榔籽提取物中具有较高含量的多酚及多糖,含量分别为(441.73±4.79)mg/g和(411.47±6.01)mg/g。动力学试验结果表明,槟榔壳及槟榔籽提取物对酶活性的抑制作用类型为竞争与非竞争的混合型抑制,而槟榔花提取物对酶活性的抑制作用类型为竞争与反竞争的混合型抑制。试验结果表明槟榔籽提取物对α-葡萄糖苷酶活性的抑制效果显著,具有很好的开发利用价值。The inhibitory effects of areca nut extracts on α-glucosidase were studied,and the dynamic properties were analyzed by lineweaver-burk double reciprocal method. Results indicated that the areca husk,seed and flower extracts were all had certain inhibitory effect on α-glycosidase,IC50 were (2.87 ±0.48) mg/mL,(1.50±0.31)μg/mL and (4.00 ±0.53) mg/mL,respectively. Inhibitory effect of areca seed extract was obviously higher than that of positive control acarbose [IC50 was (0.71±0.09) mg/mL]. By analyzing the polyphenols and polysaccharides in the extracts,it was found that the content of polyphenols and polysaccharides in the areca seed extract was higher,which was (441.73±4.79) mg/g and (411.47±6.01) mg/g,respectively. The kinetic results showed that the inhibitory effect of areca husk and areca seed extracts on α-glucosidase was a mixture of competitive inhibition and non-competitive inhibition. While the inhibitory effect of areca flower extract on α-glucosidase was a mixture of competitive and anti-competitive inhibition. The results indicated that the extracts of areca nut had significant inhibitory effect on α-glucosidase,which had good value of development and utilization.

关 键 词：槟榔 Α-葡萄糖苷酶 抑制活性 动力学 多酚多糖含量 

分 类 号：R285[医药卫生—中药学]