Bioactivity of compounds isolated from the leaves of the Lantana camara Linn plant  

作　　者：Suryati Elida Mardiah Mai Efdi Kartika MZ Yuni Mala Sari 

机构地区：[1]Department of Chemistry, Faculty of Mathematics and Sciences, Andalas University

出　　处：《Journal of Chinese Pharmaceutical Sciences》2019年第5期360-368,共9页中国药学（英文版）

摘　　要：Two compounds with different bioactivities were isolated from the leaves of the Lantana camara Linn plant.Compound 1 was triterpenoid(Lantadene B)(22β-dimethylacroyloxy-3-oxoolean-12-en-28-oic-acid,C_(35)H_(52)O_5),which was isolated from n-hexane fraction and obtained as white solid(amorphous).Compound 2 was glycoside flavonoid(5-hydroxy-6,4′-dimethoxy flavon-7-O-glucopyranose,C_(23)H_(24)O_(11)),which was isolated from ethyl acetate and obtained as pale yellow solid(amorphous).Extraction was performed with maceration method using methanol solvent.Subsequently,fractionation was carried out using n-hexane and ethyl acetate solvents.Isolation and purification of both fractions were conducted using chromatography method.The structure of the isolated compounds was determined through spectral analyses of ultraviolet(UV),infrared(IR),and nuclear magnetic resonance(~1H NMR and ^(13)C NMR),distortionless enhancement by polarization transfer(DEPT),heteronuclear multiple bond connectivity(HMBC),heteronuclear single quantum correlation(HSQC),and H-H COSY(~1H-~1H homonuclear correlated spectroscopy).The cytotoxic activity of compound 1 was tested against MCF-7 breast cancer cells in vitro using MTT assay(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide),showing very strong cytotoxic activity in inhibiting the growth of MCF-7 breast cancer cells,and the IC_(50) value was 1.1336μM.The antioxidant activity of compound 2 was tested using DPPHassay(1,1-diphenyl-2-picrylhydrazyl),showing strong activity in inhibiting free radicals,and its IC_(50) value was 71.03 mg/L.Two compounds with different bioactivities were isolated from the leaves of the Lantana camara Linn plant.Compound 1 was triterpenoid(Lantadene B)(22β-dimethylacroyloxy-3-oxoolean-12-en-28-oic-acid,C35H52O5),which was isolated from n-hexane fraction and obtained as white solid(amorphous).Compound 2 was glycoside flavonoid(5-hydroxy-6,4′-dimethoxy flavon-7-O-glucopyranose,C23H24O11),which was isolated from ethyl acetate and obtained as pale yellow solid(amorphous).Extraction was performed with maceration method using methanol solvent.Subsequently,fractionation was carried out using n-hexane and ethyl acetate solvents.Isolation and purification of both fractions were conducted using chromatography method.The structure of the isolated compounds was determined through spectral analyses of ultraviolet(UV),infrared(IR),and nuclear magnetic resonance(1H NMR and 13C NMR),distortionless enhancement by polarization transfer(DEPT),heteronuclear multiple bond connectivity(HMBC),heteronuclear single quantum correlation(HSQC),and H-H COSY(1H-1H homonuclear correlated spectroscopy).The cytotoxic activity of compound 1 was tested against MCF-7 breast cancer cells in vitro using MTT assay(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide),showing very strong cytotoxic activity in inhibiting the growth of MCF-7 breast cancer cells,and the IC50 value was 1.1336μM.The antioxidant activity of compound 2 was tested using DPPHassay(1,1-diphenyl-2-picrylhydrazyl),showing strong activity in inhibiting free radicals,and its IC50 value was 71.03 mg/L.

关 键 词：LANTANA camara LINN Lantadene B GLYCOSIDES flavonoid MCF-7 breast cell cancer CYTOTOXIC Antioxidant activity 

分 类 号：R284[医药卫生—中药学]