多糖脱蛋白方法的研究进展  

Research progress on the methods for deproteinization of polysaccharide

作　　者：何美佳 刘晓[1] 唐翠翠 曹素健 秦玲 毛文君[1,2] HE Mei-jia;LIU Xiao;TANG Cui-cui;CAO Su-jian;QIN Ling;MAO Wen-jun(Key Laboratory of Marine Drugs,Ministry of Education,Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering,School of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China;Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology,Qingdao 266237,China)

机构地区：[1]中国海洋大学海洋药物教育部重点实验室,山东省糖科学与糖工程重点实验室,医药学院,山东青岛266003 [2]青岛海洋科学与技术国家实验室海洋药物与生物制品功能实验室,山东青岛266237

出　　处：《中国海洋药物》2019年第3期82-86,共5页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金项目(41476108);国家自然科学基金委员会-山东省人民政府联合资助海洋科学研究中心项目(U1606403);青岛海洋科学与技术国家实验室鳌山科技创新计划项目(2016ASKJ08,2015ASKJ02)资助

摘　　要：多糖由于其独特的生物活性受到广泛的关注,成为近年来的研究热点之一。但是,从天然资源中提取的多糖组分常含有蛋白质杂质,而蛋白质的存在将对多糖结构和生物活性研究造成干扰。因此,采用高效、低污染、且对多糖结构和活性不造成破坏的脱蛋白方法十分重要。本文对传统的多糖脱蛋白方法和新型脱蛋白方法进行介绍。Polysaccharides have attracted considerable attention due to their unique biological activities,and have become one of the research hotspots.However,the polysaccharide extracted from natural sources often contains protein impurities,which interfere with the analyses of structure and biological activity of polysaccharide.Therefore,it is very important to utilize the deproteinizing method of polysaccharide,which is highly efficient,low in pollution,and does not destroy structure and biological activity of polysaccharide.This article reviews the traditional and novel methods for deproteinization of polysaccharide.

关 键 词：多糖 生物活性 脱蛋白 多糖回收率 

分 类 号：R914[医药卫生—药物化学]