玉米酒精糟毒素的微生物降解研究  

Study on Microbial Degradation of Toxins in Corn Alcohol Grains

作　　者：王德慧 聂志岩 王少海 张怡 童应凯 商志聪 申丹凝 田晴 魏雅婷 张乃楠 WANG Dehui;NIE Zhiyan;WANG Shaohai;ZHANG Yi;TONG Yingkai;SHANG Zhicong;SHEN Danning;TIAN Qing;WEI Yating;ZHANG Nainan(College of Agronomy and Resources and Environment,Tianjin Agricultural University,Tianjin 300384,China)

机构地区：[1]天津农学院农学与资源环境学院生物技术系

出　　处：《天津科技》2019年第6期52-55,共4页Tianjin Science & Technology

基　　金：天津市大学生创新训练计划项目“玉米酒精糟毒素的微生物降解研究”(201810061139)

摘　　要：研究旨在降解酒精糟中几种毒素(呕吐毒素、伏马菌素、黄曲霉毒素),为酒精糟制成饲料奠定基础。利用实验得到的一株能够很好降解玉米酒糟毒素菌种酵母19-1,以酒精糟和麸皮为发酵底物进行固态发酵,通过正交试验对固态发酵过程中添加的营养条件进行研究,测得发酵物毒素的含量,并通过重复最佳配方实验对结果进行验证。综合3种毒素测得情况,确定酵母19-1降解玉米酒精糟毒素的最优搭配组合为硫酸铵0%、水12 m L、硫酸镁0.015%、磷酸二氢钾0.05%、葡萄糖0%。The purpose of this study is to degrade several toxins(vomiting toxin, fumonisin and aflatoxin) in alcohol grains,and to lay a foundation for the preparation of feed from alcohol grains. A strain of yeast 19-1, which can degrade toxins in corn alcohol grains, was obtained by strain screening. Alcohol grains and bran were used as fermentation substrates for solid-state fermentation. The nutrient conditions added in solid-state fermentation were studied by orthogonal experiment, and the content of toxin was determined. The results were verified by repeated optimal formula experiments. The best matching combination of yeast 19-1 for degrading the toxins in corn alcohol grains was 0% of ammonium sulfate, 12 ml of water, 0.015% of magnesium sulfate, 0.05% of potassium dihydrogen phosphate and 0% of glucose.

关 键 词：玉米酒精糟 呕吐毒素 伏马菌素 黄曲霉毒素 培养基优化 

分 类 号：S635.3[农业科学—蔬菜学] TS255.3[农业科学—园艺学]