灵芝免疫调节蛋白LZ-8及云芝免疫调节蛋白FIP-tvc对几种癌细胞系的抑制活性检测  

Analysis of Inhibitory Effect of Fungal Immunomodulatory Protein LZ-8 from Ganoderma lucidum and FIP-tvc from Trametes versicolor to Several Cancer Cell Lines

作　　者：王雪妍[1] 周伏忠[1,2] 向凌云[2] 陈晓飞[2] 宁萌[2] 刁文涛[1,2] WANG Xueyan;ZHOU Fuzhong;XIANG Lingyun;CHEN Xiaofei;NING Meng;DIAO Wentao(Key Laboratory of Microbial Engineering of Henan Province,Zhengzhou 450008,China;Institute of Biology Co. Ltd.,Henan Academy of Sciences,Zhengzhou 450008,China)

机构地区：[1]河南省微生物工程重点实验室,郑州450008 [2]河南省科学院生物研究所有限责任公司,郑州450008

出　　处：《河南科学》2019年第6期914-918,共5页Henan Science

基　　金：河南省科学院2018重大科技突破专项(18ZP05001);河南省科学院基本科研业务费(18JK16011);河南省科学院助推科技成果转化专项(18ZT05017)

摘　　要：在体外培养的人非小细胞肺癌A549细胞、人胃腺癌BGC-823细胞、人结肠癌HCT116细胞、人肝癌Hep3B细胞、人宫颈癌Hela细胞中加入不同浓度的酵母异源表达的LZ-8或FIP-tvc,培养24h后显微镜下观察细胞形态变化,并用CCK8试剂盒检测细胞存活率,以用来检测灵芝免疫调节蛋白LZ-8及云芝免疫调节蛋白FIP-tvc对几种癌细胞生长抑制能力.结果显示,LZ-8和FIP-tvc在5~10μg/mL浓度时使A549、BGC-823、Hela和Hep 3B细胞贴壁性变差,且CCK8结果显示前3种细胞的存活率有不同程度的降低(80%~95%存活率),而在20μg/mL浓度时FIP-tvc对Hep 3B细胞的存活率开始有明显抑制效果(89%存活率),但对HCT116细胞的形态无影响,对其存活率也无抑制作用.In this paper,the morphological changes of human non-small cell lung cancer cell line A549,human gastric adenocarcinoma cell line BGC-823,human colon cancer cell line HCT 116,human liver cancer cell line Hep 3B and human cervical carcinoma cell line Hela were observed after culturing with LZ-8 or FIP-tvc for 24 hours, and the viability of these cell lines was further analyzed with CCK8 kit to analyze the inhibitory effect of LZ-8 from Ganoderma lucidum and FIP-tvc from Trametes versicolor to these cancer cell lines. The results showed that the cell adherence of A549,BGC-823,Hela and Hep 3B was significantly inhibited by LZ-8 and FIP-tvc at 5-10 μg/mL, and the viability of the first three cell lines was simultaneously inhibited and analyzed with CCK8 kit(80%-95%), but the inhibitory effect of FIP-tvc to the viability of Hep 3B was appeared when the concentration was reached to 20 μg/mL(89%). While morphology of HCT 116 was not changed and its viability was also not inhibited by LZ-8 or FIP-tvc at any test concentrations.

关 键 词：灵芝免疫调节蛋白 云芝免疫调节蛋白 抑癌活性 癌细胞存活率 

分 类 号：Q78[生物学—分子生物学] R392.1[医药卫生—免疫学]