不同的萃取方式分析葫芦巴浸膏挥发性成分  

Analysis of Volatile Components of Fenugreek Extract via Different Extraction Methods

作　　者：董振山[1] 黄世杰 黄善松 李庆祥[1] 李小兰 李志华 胡志忠 张峻松[1] Zhen-shan;HUANG Shijie;HUANG Shan-song;LI Qing-xiang;LI Xiao-lan;LI Zhi-hua;HU Zhi-zhong;ZHANG Jun-song(1.College of Food andBioengineering,Zhengzhou University of Light Industry,Zhengzhou 450002,China;Technology Center,Guangxi China Tobacco Co.,Ltd.,Nanning 530001,China)

机构地区：[1]郑州轻工业学院食品与生物工程学院,河南郑州450002 [2]广西中烟有限责任公司技术中心,广西南宁530001

出　　处：《化学试剂》2019年第6期608-613,共6页Chemical Reagents

基　　金：广西中烟工业有限责任公司资助项目(201745-000034012)

摘　　要：为了明确葫芦巴浸膏中挥发性成分组成、含量及不同萃取方式对葫芦巴浸膏挥发性成分萃取效果的影响,分别采用固相微萃取(SPME)、溶剂萃取(LLE)和同时蒸馏萃取(SDE)对葫芦巴浸膏中挥发性成分进行提取,利用GC-MS技术对挥发性成分进行分析鉴定。3种萃取方法共鉴定出160种挥发性成分,其中固相微萃取(SPME)37种、溶剂萃取(LLE)66种、同时蒸馏萃取(SDE)113种,3种方式共有的16种,具有葫芦巴浸膏特征香味成分的葫芦巴内酯3种方式均能萃取出来,比较3种萃取方法可知:同时蒸馏萃取(SDE)和溶剂萃取(LLE)对葫芦巴浸膏中香味物质提取的更为全面,两种萃取方式共鉴定出151种。In order to clarify the composition and content of volatile components in the fenugreek extract and the effect of different extraction methods on the extraction of volatile components of fenugreek extract,the volatile components in the fenugreek extract were extracted by solid phase microextraction(SPME),solvent extraction(LLE)and simultaneous distillation extraction(SDE),and the volatile components were analyzed and identified by GC-MS.A total of 160 volatile components were identified by three extraction methods,including 37 solid phase microextraction(SPME),66 solvent extraction(LLE),and 113 kinds of simultaneous distillation(SDE).The fenugreek lactone characteristic of the fenugreek extract can be extracted in three ways.Comparing the three extraction methods,it can be known that the simultaneous extraction(SDE)and solvent extraction(LLE)extract the aroma from the fenugreek extract more comprehensive,and a total of 151 species were identified by two extraction methods.

关 键 词：葫芦巴浸膏 溶剂萃取 固相微萃取 同时蒸馏萃取 GC-MS 

分 类 号：O657.7[理学—分析化学]