Detection of Gram-Negative Bacteria Carrying the blaKPC-2 Gene from Mangrove Sediments  

作　　者：Wallace Ribeiro Nunes Neto Jessica Mayara Mendes Araujo Darlan Ferreira da Silva Joveliane de Melo Monteiro Wallace Borges Pachêco Jessflan Rafael Nascimento Santos Maria Raimunda Chagas Silva Maria Rosa Quaresma Bomfim Andrea de Souza Monteiro 

机构地区：[1]Laboratory of Applied Microbiology,CEUMA University,Sao Luis Maranhao 65000-000,Brazil [2]Laboratory of Environmental Sciences,CEUMA University,Sao Luis Maranhao 65000-000,Brazil [3]Laboratory of Molecular Biology Sciences,CEUMA University,Sao Luis Maranhao 65000-000,Brazil

出　　处：《Journal of Pharmacy and Pharmacology》2019年第8期485-492,共8页药剂与药理学（英文版）

摘　　要：Mangroves are environments that have fast cycles associated with high concentration of bacterial decomposers.These are impacted by anthropogenic pollution due to contamination of bacterial species carrying resistance genes.This study aims to evaluate the metabolic profile of the microbiota in mangrove sediments,and verify the presence of Gram-negative bacteria resistant to meropenem.The samples were obtained from location along the Anil river and were seeded in medium supplemented with the antibiotic meropenem in increasing concentrations.The DNA was analyzed by multiplex PCR for detecting resistance genes forβ-lactam antibiotics.The bacteria were identified by Matrix-assisted laser desorption ionization–time of flight mass spectrometry(MALDI-TOF MS).The genes were sequenced by ABI PRISM 3100,analyzed by MEGA 6.0 program,and the sequence identified was assessed by GenBank using the BLAST algorithm.Ecoplate■the kit was used to determine the metabolic profile of the microbiota.The results of the six bacterial isolates showed the blaKPC-2 gene and were identified as Stenotrophomonas maltophilia and Pseudomonas putida.The samples showed a greater diversity Shannon index,a rich substrate consumption and high equity.There was a metabolic richness such as carbon consumption profiles,being a factor of adaptation of pathogenic bacteria carrying resistance genes toβ-lactamics antibiotics.

关 键 词：Antibiotic RESISTANT MEROPENEM BACTERIAL ISOLATES 

分 类 号：R[医药卫生]