QuEChERS前处理结合高效液相色谱-串联质谱法同时测定酵母产品中4种抗氧化剂和5种防腐剂  

Simultaneous determination of antioxidants and p-hydroxybenzoate antiseptics in yeast products using QuEChERS and high-performance liquid chromatography-tandem mass spectrometry

作　　者：李艳美 李芳 粟有志 周均 罗琼 王静文 王兴磊 LI Yanmei;LI Fang;SU Youzhi;ZHOU Jun;LUO Qiong;WANG Jingwei;WANG Xinglei(Key Laboratory of Pollutant Chemistry and Environmental Treatment,YiLi Normal University,Yining 835000,China;Technical Centre of Yining Customs,Yining 835000,China)

机构地区：[1]伊犁师范大学,污染物化学与环境治理重点实验室,新疆伊宁835000 [2]伊宁海关技术中心,新疆伊宁835000

出　　处：《食品与发酵工业》2019年第12期215-221,共7页Food and Fermentation Industries

基　　金：伊犁师范大学污染物化学与环境治理重点实验室开放课题(2018HJZD01)

摘　　要：建立高效液相色谱-串联质谱法(high performance liquid chromato graphy-tanelem mass spectrometry,HPLC-MS/MS)法同时测定酵母产品中4种抗氧化剂和5种对羟基苯甲酸酯类防腐剂的定性定量分析方法。样品经溶解脱脂,用正己烷饱和的乙腈提取,浓缩至干流动相复溶,经C 18净化、过膜后供仪器测试。采用电喷雾离子源、多重反应监测模式检测,以保留时间和特征离子对比较定性定量。9种分析物在0.1~10 mg/L相关系数(r^2)均大于0.992,方法定量限为0.10~0.50 mg/kg,酵母产品中3水平6平行的加标回收实验,回收率在78.9%~108.6%,相对标准偏差为4.2%~15.8%。该方法快速简便、准确可靠,适用于酵母产品中4种抗氧化剂和5种对羟基苯甲酸酯类防腐剂的测定,为高通量检测此类物质提供了技术支持。A qualitative and quantitative method based on high performance liquid chromatography-tandem mass spectrometry for simultaneous determination of four antioxidants and five p-hydroxybenzoate antiseptics in yeast products was developed.Samples were defatted and extracted with acetonitrile-hexane mixture,and then concentrated to dry and redissolved in the mobile phase.The solution was cleaned by C 18 powder adsorption and filtered.There were nine analytes determined by using electrospray ion source in multiple reaction monitoring mode.The results showed that all analytes had good linearity in the range of 0.1-10 mg/L with correlation coefficients(r^2)larger than 0.992,and the quantitation limits of the method were in the range of 0.10-0.50 mg/kg,and the average recovery rate was 78.9%-108.6%,and RSDs were 4.2%-15.8%.This convenient and accurate method provides technical supports for detecting such substances based on high throughput technology.

关 键 词：防腐剂 抗氧化剂 酵母产品 高效液相色谱-串联质谱法 

分 类 号：O657.63[理学—分析化学] TQ207.3[理学—化学]