响应面法优化太空茄子叶总黄酮的提取工艺  

Optimization of extraction process for total flavonoids from space eggplant leaves by response surface methodology

作　　者：杨彬[1] 金小青 李彩霞[1] 焦扬[1] YANG Bin;JIN Xiao-qing;LI Cai-xia;JIAO Yang(College of Agriculture and Biotechnology,Hexi University,Zhangye 734000,China;Key Laboratory of Hexi Corridor Resources Utilization of Gansu,Zhangye 734000,China)

机构地区：[1]河西学院农业与生物技术学院,甘肃张掖734000 [2]甘肃省高校河西走廊特色资源利用省级重点实验室,甘肃张掖734000

出　　处：《甘肃农业大学学报》2019年第3期163-170,共8页Journal of Gansu Agricultural University

基　　金：国家大学生创新创业训练计划项目(201510740001);甘肃省科技支撑计划-农业类(144NKCA241)

摘　　要：【目的】优化太空茄子叶中总黄酮的提取工艺.【方法】以太空茄子叶为试材,在单因素试验的基础上,取乙醇体积分数、料液比、提取时间为自变量,太空茄子叶总黄酮得率为响应值,以响应面法得到二次多项式模型,优化其提取条件,并采用HPLC对总黄酮进行分析和鉴定.【结果】在温度(80 ℃)固定的条件下,太空茄子叶总黄酮的最佳工艺条件为:提取时间1.8 h、料液比1 ∶ 42(g/mL)、乙醇体积分数78%,在此条件下,太空茄子叶总黄酮得率为9.82%,与预测值9.87%相吻合.【结论】响应面分析法优化太空茄子叶总黄酮的提取工艺合理可行.【Objective】 To optimize the extraction process of total flavonoids from space eggplant leaves.【Method】 On the basis of a single factor experiment,space eggplant leaves were used as the test material,ethanol concentration,solid-to-liquid ratio,and extraction time were used as independent variables,the extraction yield of total flavonoids was used as response value.A quadratic polynomial model was established according to the response surface methodology.By optimizing the extracting condition,the total flavonoids were analyzed and identified by HPLC.【Result】 At the constant ambient temperature of 80 ℃,the optimal conditions for ethanol reflux extraction of total flavonoids were found to be as follows,extraction time 1.8h,solid-to-liquid ratio 1 ∶ 42(g/mL),ethanol concentration 78%.Under these conditions,the extraction yield of total flavonoids was 9.82%,which was fully in agreement with the predicted value of 9.87%.【Conclusion】 It is suggested that response surface analysis to optimazation of extraction process of total flavenoids from space eggplant leaves is feasible.

关 键 词：太空茄子叶 黄酮 响应面法 提取工艺 

分 类 号：TQ041[化学工程]