过氧化氢处理中鼠伤寒沙门氏菌VBNC态形成及其机制解析  

Exploration of the formation and mechanism of VBNC state of Salmonella enterica serovar Typhimurium during hydrogen peroxide disinfection

作　　者：聂新颖 廖红梅[1] 刘元法[1] NIE Xin-ying;LIAO Hong-mei;LIU Yuan-fa(School of Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China)

机构地区：[1]江南大学食品学院

出　　处：《食品与机械》2019年第7期67-73,共7页Food and Machinery

基　　金：国家重点研发计划(编号：2017YFD0400703-3);国家自然科学基金(编号：31471714);中国博士后科学基金资助项目(编号：2017M621632)

摘　　要：为确定过氧化氢的有效杀菌浓度,以鼠伤寒沙门氏菌为目标微生物,借助分子生物学手段,探究过氧化氢对自来水中鼠伤寒沙门氏菌活性和可培养性的影响及其活的非可培养状态(viable but non-culturable, VBNC)的形成原因。结果表明:较低过氧化氢浓度(0~15 mmol/L)处理可达到部分杀菌的效果,1 mol/L以上过氧化氢可完全灭菌。而15 mmol/L 过氧化氢处理导致4.08 lg(CFU/mL)鼠伤寒沙门氏菌全部进入VBNC状态。该状态的沙门氏菌形态和结构改变,过氧化氢酶、超氧化物歧化酶和谷胱甘肽过氧化物酶几乎完全被钝化,处理中产生的自由基强度在0~30 min内与VBNC发生系数呈正相关。In order to determine the effective bactericidal concentration of hydrogen peroxide, Salmonella enterica serovar Typhimurium was used as a target microorganism to explore the effect of hydrogen peroxide on activity and culturability of it in tap water and its viable but non-culturable (VBNC) state by means of molecular biology. The results showed that lower hydrogen peroxide concentration (0~15 mmol/L) could kill some S . Typhimurium, which could be completely sterilized with 1 mol/L hydrogen peroxide. It was also found that 4.08 lg(CFU/mL) S . Typhimurium could be induced into a VBNC state as exposure to 15 mmol/L hydrogen peroxide. The morphology and structure of S . Typhimurium in VBNC state have changed, and the catalase, superoxide dismutase and glutathioneperoxidase were almost completely inactivated. It was detected that the intensity of free radicals generated during treatment was positively correlated with VBNC indexes within 0~30 min.

关 键 词：过氧化氢 鼠伤寒沙门氏菌 活的非可培养状态 自由基 

分 类 号：TS201.3[轻工技术与工程—食品科学]