核桃粕中环烯醚萜苷提取分离与抗疲劳活性研究  

Extraction,identification and anti-fatigue activity of iridoid glycosides from walnut meal

作　　者：金蒙 徐德平[1] 詹积 陈晨[1] JIN Meng;XU De-ping;ZHAN Ji;CHEN Chen(Food Faculty of Jiangnan University, Wuxi, Jiangsu 214122, China)

机构地区：[1]江南大学食品学院

出　　处：《食品与机械》2019年第7期172-175,236,共5页Food and Machinery

基　　金：江苏高校品牌专业建设工程资助项目(编号：PPZY2015A052);云南省重大科技专项计划项目(编号：2018ZG003)

摘　　要：核桃粕经乙醇提取,乙酸乙酯、正丁醇分别萃取,不同溶剂萃取物通过小鼠跑台试验和负重游泳试验确定抗疲劳作用,并对有明确抗疲劳效果的萃取物进行MCI分离柱、ODS反相柱层析分离,得到的单体化合物采用核磁共振法鉴定其结构。结果表明:核桃粕醇提物正丁醇萃取物抗疲劳作用效果最为明显,该部分上ODS柱经5%乙醇洗脱分离得化合物Ⅱ,鉴定得该化合物为4-羧基-7-羟基-8-乙酸乙酯基-3,6-环戊烯环烯醚萜苷,且能显著提高小鼠运动抗疲劳能力。The extraction and identification and anti-fatigue activity of iridoid glycosides in walnut meal were studied. The walnut was extracted by ethanol reflux, extracted with ethyl acetate and n-butanol. Different solvent extracts were used to determine the anti-fatigue effect by mouse running test and weight-bearing swimming test. The extract with clear anti-fatigue effect was further separated by MCI and ODS reverse-phase column chromatography, and the obtained monomer compound was identified by NMR. The results showed that the extract of n-butanol from the walnut meal extract had the most obvious effect on the anti-fatigue effect of the mice. The part of the ODS column was eluted with 5% ethanol to obtain the compound II. The compound was identified as the iridoid glycoside. And can significantly improve the anti-fatigue ability of mice.

关 键 词：核桃粕 环烯醚萜苷 抗疲劳 分离 结构鉴定 

分 类 号：TS229[轻工技术与工程—粮食、油脂及植物蛋白工程]