Identification and developmental expression of putative gene encoding juvenile hormone esterase(CpJHE-like) in codling moth, Cydia pomonella(L.)  

作　　者：HUANG Cong WU Qiang JIANG Chun-yan XING Long-sheng SHI Guo-liang ZHANG Bin QIAN Wan-qiang LI You-zhi XI Yu YANG Nian-wan WAN Fang-hao 

机构地区：[1]College of Plant Protection,Hunan Agricultural University,Changsha 410128,P.R. China [2]State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,P.R. China [3]Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,P.R. China [4]College of Plant Health and Medicine,Qingdao Agricultural University,Qingdao 266109,P.R.China

出　　处：《Journal of Integrative Agriculture》2019年第7期1624-1633,共10页农业科学学报（英文版）

基　　金：supported by the National Key Research and Development Program of China(2016YFC1201200,2017YFC1200600 and 2016YFC1200602);the Basic Research on Science and Technology Project of Shenzhen,China(JCYJ20160530191934833);the Dapeng New District Industrial Development Special Fund of Shenzhen,China(KY20180215)

摘　　要：Juvenile hormone esterase(JHE) is a key enzyme for insects,playing an important role in the regulation of insect growth,development,diapause and reproduction.We identified a complete putative JHE of Cydia pomonella(CpJHE-like) which is comprised of a 1 761 bp coding sequence(CDS) encoding 587 amino acid residues from the transcriptome data.The deduced protein sequence of CpJHE-like showed the highest identity of 60.44% with the Adoxophyes honmai JHE(AhJHE) and the minimal identity of 25.81% with Aedes aegypti JHE(AaJHE).CpJHE-like exhibited all the seven typical motifs of the functional JHEs and had the highly consistent tertiary structure with Manduca sexta JHE(MsJHE).Phylogenetic analysis showed that the CpJHE-like was close to two JHEs from the family Tortricidae.The CpJHE-like transcript level take a leap in the 3-day-old fifth instar larva,increased about 300-fold compared to the basal level.Tissue-specific expression profile showed that the CpJHE-like transcript was expressed mainly in the fat body.This study indicates that the CpJHE-like is the functional JHE,which may play vital roles in the development and reproduction of C.pomonella.Juvenile hormone esterase(JHE) is a key enzyme for insects, playing an important role in the regulation of insect growth, development, diapause and reproduction. We identified a complete putative JHE of Cydia pomonella(CpJHE-like) which is comprised of a 1 761 bp coding sequence(CDS) encoding 587 amino acid residues from the transcriptome data. The deduced protein sequence of CpJHE-like showed the highest identity of 60.44% with the Adoxophyes honmai JHE(AhJHE) and the minimal identity of 25.81% with Aedes aegypti JHE(AaJHE). CpJHE-like exhibited all the seven typical motifs of the functional JHEs and had the highly consistent tertiary structure with Manduca sexta JHE(MsJHE). Phylogenetic analysis showed that the CpJHE-like was close to two JHEs from the family Tortricidae. The CpJHE-like transcript level take a leap in the 3-day-old fifth instar larva, increased about 300-fold compared to the basal level. Tissue-specific expression profile showed that the CpJHE-like transcript was expressed mainly in the fat body. This study indicates that the CpJHE-like is the functional JHE, which may play vital roles in the development and reproduction of C. pomonella.

关 键 词：JUVENILE HORMONE ESTERASE CpJHE-like codling MOTH Cydia pomonella 

分 类 号：S[农业科学]