剧毒卡尔藻与东海原甲藻间的化感作用研究  

ALLELOPATHIC INTERACTION OF TWO DINOFLAGELLATES: KARLODINIUM VENEFICUM AND PROROCENTRUM DONGHAIENSE

作　　者：王蕊 黄文雯[1] 吴佳俊[1,2] 陈荔 王江涛[3] WANG Rui;HUANG Wen-Wen;WU Jia-Jun;CHAN Lai-Leo;WANG Jiang-Tao(Shenzhen Key Laboratory for the Sustainable Use of Marine Biodiversity,Research Centre for the Oceans and Human Health,City University of Hong Kong Shenzhen Research Institute,Shenzhen 518000,China;The State Key Laboratory of Marine Pollution,City University of Hong Kong,Hong Kong SAR 999077,China;College of Chemistry and Chemical Engineering,Ocean University of China,Qingdao 266100,China)

机构地区：[1]香港城市大学深圳研究院海洋与人类健康研究中心,深圳市海洋生物多样性可持续利用重点实验室,深圳518000 [2]香港城市大学海洋污染国家重点实验室,香港999077 [3]中国海洋大学化学化工学院,青岛266100

出　　处：《海洋与湖沼》2019年第4期858-863,共6页Oceanologia Et Limnologia Sinica

基　　金：中国博士后科学基金项目,2018M633157号;深圳市知识创新计划基础研究项目,JCYJ20170818103413138号

摘　　要：为研究剧毒卡尔藻(Karlodinium veneficum)与东海原甲藻(Prorocentrum donghaiense)之间的化感作用,在f/2营养盐条件下,分别采用了混合培养及滤液培养的方法。结果表明:剧毒卡尔藻指数生长后期的无藻细胞滤液明显抑制了东海原甲藻的生长,而东海原甲藻滤液对剧毒卡尔藻的生长几乎无影响,化感作用作为重要的影响因素使剧毒卡尔藻获得竞争优势。将剧毒卡尔藻指数生长末期的无藻细胞滤液置于固相萃取(SPE)C18小柱,经吸附、不同比例的甲醇溶液洗脱,考察不同洗脱部分对东海原甲藻生长的影响,发现80%的甲醇洗脱部分明显抑制了东海原甲藻的生长。本研究为了解剧毒卡尔藻的竞争机制提供了重要的科学依据,今后将重点分析剧毒卡尔藻释放的有效抑制东海原甲藻生长的化感物质。Potential allelopathic interaction between Karlodinium veneficum and Prorocentrum donghaiense was investigated in bi-algal culture and culture filtrate in nutrient-replete medium.The results show that the cell-free filtrate of the K.veneficum culture in later exponential growth phase inhibited the growth of P.donghaiense apparently.In contrast,the filtrate of P.donghaiense exerted no allelopathic activity to K.veneficum,demonstrating that the release of allelopathic compounds inhibited the growth.Furthermore,the cultures of K.veneficum in later exponential growth phase were harvested and loaded onto a RP column using C18,and eluted with a step gradient consisting of different percentage of MeOH.The results show that 80%MeOH fraction depressed P.donghaiense growth more obviously.Further studies are needed to explore allelochemicals released by K.veneficum.

关 键 词：种间竞争 化感作用 剧毒卡尔藻 东海原甲藻 

分 类 号：X173[环境科学与工程—环境科学]