淮安市禽畜肉中金黄色葡萄球菌污染及其病原学特征分析  

Prevalence and characterization of Staphylococcus aureus in meats of animal origin in Huai’an

作　　者：李兵兵[1] 刘靓[1] 李双姝 金晶[1] 刘纯成[1] 赵怀荣[1] LI Bingbing;LIU Liang;LI Shuangshu;JIN Jing;LIU Chuncheng;ZHAO Huairong(Huai'an Center for Disease Control and Prevention ,Jiangsu Huai'an 223001,China)

机构地区：[1]淮安市疾病预防控制中心

出　　处：《中国食品卫生杂志》2019年第3期217-221,共5页Chinese Journal of Food Hygiene

基　　金：江苏省预防医学科研课题(Y2018047)

摘　　要：目的了解淮安市市售禽畜肉中金黄色葡萄球菌的污染状况,并研究分离菌株的病原学特征。方法 2017-2018年从淮安市8个县区的超市和农贸市场采集禽畜肉,分离金黄色葡萄球菌。琼脂扩散法分析分离菌株的耐药特性,聚合酶链式反应法检测毒力基因携带情况。结果 378份禽畜肉中有60份样品检出金黄色葡萄球菌,总检出率为15.87%。不同禽畜肉中金黄色葡萄球菌检出率由高到低依次为鸭肉(22.50%,18/80)、鸡肉(17.95%,14/78)、牛肉(15.38%,10/65)、猪肉(12.94%,11/85)和羊肉(10.00%,7/70)。金黄色葡萄球菌分离菌株对克林霉素耐药率最高(71.67%,43/60),对万古霉素均敏感,多重耐药菌株占63.33%(38/60),耐甲氧西林金黄色葡萄球菌占23.33%(14/60)。肠毒素基因sea、seb、sec、sed、see的携带率分别为6.67%(4/60)、6.67%(4/60)、23.33%(14/60)、48.33%(29/60)、10.00%(6/60),中毒休克综合征毒素-1基因tsst-1的携带率为5.00%(3/60)。耐药基因ant(4′,4″)、mecA、vanA、tetM检出率分别为88.33%(53/60)、13.33%(8/60)、11.67%(7/60)和8.33%(5/60)。结论淮安市市售禽畜肉中金黄色葡萄球菌的污染率较高,毒力基因携带率高,耐药状况较重,提示存在潜在的食品安全隐患。Objective To investigate the contamination and characterization of Staphylococcus aureus in meats of animal origin in Huai'an. Methods 378 meat samples of animal origin were collected from 8 counties in Huai'an during 2017 to 2018 and were tested for S. aureus. The agar diffusion method was used in antimicrobial susceptibility test and polymerase chain reaction ( PCR) was used to detect the S. aureus enterotoxin genes. Results Of the total 378 samples, 60 samples (15. 87%) were positive for S. aureus. And the positive rate of duck, chicken, beef, pork and mutton were 22. 50%(18/80), 17.95%( 14/78), 15.38%(10/65), 12. 94%( 11/85) and 10. 00%(7/70), respectively. The antimicrobial susceptibility test indicated that S. aureus isolates showed the highest resistance to clindamycin (71. 67%, 43/60) and were all sensitive to vancomycin. Multidrug resistant strains accounted for 63. 33%( 38/60) and the methicillin resistant S. aureus strains accounted for 23. 33%( 14/60). The carriage rate of enterotoxin gene sea, seb, sec, sed and see were 6. 67%(4/60), 6. 67%(4/60), 23. 33%( 14/60), 48. 33%( 29/60) and 10. 00%(6/60), respectively. And the carriers of toxic shock syndrome toxin-1 gene tsst-1 was 5. 00%( 3/60). The detection rates of drug resistance gene ant ( 4', 4"), mecA, vanA and tetM were 88. 33%( 53/60), 13. 33%( 8/60), 11. 67%( 7/60 ) and 8. 33%( 5/60), respectively. Conclusion The contamination of S. aureus in meats of animal origin in Huai'an was serious, and the isolates showed high antibiotic resistance and high carriage rate of enterotoxin genes, which may result in potential risks to food safety.

关 键 词：金黄色葡萄球菌 耐药性 毒力基因 食源性致病菌 禽畜肉 

分 类 号：R155[医药卫生—营养与食品卫生学]