酵解姬松茸多糖对小鼠免疫指标和肠道菌群的影响  

Effects of glycolytic polysaccharides of Agaricus blazei Murrill on immune indexes and intestinal flora in mice

作　　者：申秀娟 张磊[1] 杨美玉 杨硕 王莹[1] 李凯琳 李大军[1] SHEN Xiujuan;ZHANG Lei;YANG Meiyu;YANG Shuo;WANG Ying;LI Kailin;LI Dajun(College of Food Science and Engineering,Jilin Agricultural University,Changchun 130118)

机构地区：[1]吉林农业大学食品科学与工程学院

出　　处：《食品科技》2019年第6期215-220,共6页Food Science and Technology

基　　金：吉林省科技厅重点项目(20150204035NY)

摘　　要：目的:采用小鼠试验检验酵解姬松茸多糖对小鼠免疫和肠道菌群的影响。方法:昆明系小鼠100只,随机分为5组,分别投喂试验饲料处理:基础饲料中添加质量分数为0%、0.15%、0.3%、0.6%剂量的酵解姬松茸多糖和0.6%的水提姬松茸多糖,酵解松茸多糖来源于酵素酵解姬松茸法提取。其中各组小鼠的半数于饲喂试验第7天处死,采用平板计数法检测小鼠盲肠中乳杆菌(Lactobacilli)、双歧杆菌(Bi?dobacteria)、肠杆菌(Enterobacteria)、肠球菌(Enterococci)的数量;另一半于饲喂试验的第14天处死,测定小鼠体质量、脏器指数、血清学指标及肠道菌群数量。另准备10只小鼠于第0天处死,测定试验起始点肠道菌数量作为参照。结果:试验14 d,对小鼠脾脏指数影响顺序:酵解姬松茸多糖处理组>水提姬松茸多糖处理组>0%剂量组,前面2组与0%剂量组比较差异显著。与0%剂量组比较,酵解姬松茸多糖各处理组血清中DAO、D-LA、ET和TNF-α的水平均显著降低;0.6%剂量酵解姬松茸多糖组TNF-α含量极显著(P<0.01)低于0.6%剂量水提姬松茸多糖组,D-LA、ET显著降低(P<0.05)。试验期间,多糖处理各组肠道菌数量,与0%剂量组比较均具有差异,并与试验起始期不同;与0.6%剂量水提水提姬松茸多糖组比较,0.3%剂量酵解姬松茸多糖组肠道中乳杆菌数量极显著(P<0.01)增加;0.6%剂量酵解姬松茸多糖组双歧杆菌的数量显著(P<0.05)增加,且肠杆菌、肠球菌的数量显著(P<0.05)降低。结论:酵解姬松茸多糖具有与水提姬松茸多糖相似或更好地调节小鼠免疫功能和优化肠道菌群的作用。Objective: The effects of glycolytic polysaccharides(GP) of Agaricus blazei Murrill on immunity and intestinal flora in mice were examined. Method: One hundred Kunming mice were randomly separated to 5 groups and accordingly fed with treatment feed: basal diet separately added with glycolytic polysaccharides of Agaricus blazei Murrill in 0%,0.15%,0.3% and 0.6%, and water extracted polysaccharides(WP) of that in 0.6%. Half of the mice in each group were sacrificed on the 7 th day of the feeding test. The number of Lactobacillus, Bifidobacterium, Enterobacter, and Enterococcus in the cecum of the mouse was detected by plate counting method, and the other half was sacrificed on the 14 th day of the feeding test. The body weight, viscera index, serological index and number of intestinal flora were determined. Another 10 mice were sacrificed only at the 0 th day, and the number of intestinal bacteria at the starting point of the test was measured as a reference. Result: At the 14 d, there is significantly(P<0.05) effect of mice spleen index in treatment groups in ordinance of: GP>WP>0% treatment, except none effect impact of other viscera indexes. Compared with the 0% dose group of polysaccharides, the levels of DAO, D-LA, ET and TNF-α in the serum of Agaricus blazei GP treatment group were significantly lower;the content of TNF-α in 0.6% dose GP group of Agaricus blazei Murrill was significanty(P<0.01)lower than that in 0.6% dose WP group, the D-LA and ET were significantly decreased(P<0.05).During the experiment, the number of intestinal bacteria in each group treated with polysaccharide was different from that in the 0% dose group, and it was different from the initial period of the experiment;compared with the 0.6% dose water extracting Agaricus blazei polysaccharide group. The number of Lactobacillus in gut of the 0.3% dose Agaricus blazei GP group was significantly increased(P<0.01);the number of Bifidobacteria in the 0.6% dose group was significantly increased(P<0.05), and the number of Enterobacter and Enter

关 键 词：酵解多糖 姬松茸 免疫 肠道菌群 脏器指数 

分 类 号：TS201.2[轻工技术与工程—食品科学]