Controllable and flexible cellular network for virus cell-to-cell spread  

作　　者：Qiong Yan Cheng Wang Ji Wang Liangjun Pan Zhiling Zhang 

机构地区：[1]Key Laboratory of Analytical Chemistry for Biology(Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University

出　　处：《Chinese Chemical Letters》2019年第6期1229-1232,共4页中国化学快报（英文版）

基　　金：the National Science and Technology Major Project of China(No. 2018ZX10301405);the National Natural Science Foundation of China(Nos. 21775111, 21475099)

摘　　要：Virus spread is closely related to pathogenesis.Traditional research methods of virus spread do not distinguish cell-to-cell spread from cell-free spread.The uncertainty of virus induced plaque and virus spread direction makes it difficult to track the spread of virus in situ.Herein,the cellular network was fabricated on the basis of PEG modification and soft lithography.Therein,cell numbers and spatial distributions we re p recisely controlled,e.g.,cellular netwo rks like "WHU" can be achieved.Furthermore,a micro-injector was combined with the cellular network for virus spread on the fixed point,in which virus spread direction was limited in one dimension.The results suggested that controllable and flexible cellular network can be constructed on the PEG pattern.The synergia of micro-injector and cellular network provides an advanced tool to investigate virus cell-to-cell spread at the initial infection stage.Virus spread is closely related to pathogenesis.Traditional research methods of virus spread do not distinguish cell-to-cell spread from cell-free spread.The uncertainty of virus induced plaque and virus spread direction makes it difficult to track the spread of virus in situ.Herein,the cellular network was fabricated on the basis of PEG modification and soft lithography.Therein,cell numbers and spatial distributions we re p recisely controlled,e.g.,cellular netwo rks like "WHU" can be achieved.Furthermore,a micro-injector was combined with the cellular network for virus spread on the fixed point,in which virus spread direction was limited in one dimension.The results suggested that controllable and flexible cellular network can be constructed on the PEG pattern.The synergia of micro-injector and cellular network provides an advanced tool to investigate virus cell-to-cell spread at the initial infection stage.

关 键 词：Cellular network CELL-TO-CELL SPREAD Micro-injector PSEUDORABIES VIRUS PEG pattern 

分 类 号：O6[理学—化学]