集胞藻Synechocystis sp. PCC 6803 slr1501的克隆及原核表达分析  

Cloning and Prokaryotic Expression Analysis of slr1501 from Synechocystis sp. PCC 6803

作　　者：张燕[1] 岳寿松[1] 陈高[1] 游银伟[1] 钟怀荣 于金慧[1] Zhang Yan;Yue Shousong;Chen Gao;You Yinwei;Zhong Huairong;Yu Jinhui(Biotechnology Research Center,Shandong Academy of Agricultural Sciences/Shandong Provincial Key Laboratory of Crop Genetic Improvement,Ecology and Physiology,Jinan 250100,China)

机构地区：[1]山东省农业科学院生物技术研究中心/山东省作物遗传改良与生理生态重点实验室

出　　处：《山东农业科学》2019年第7期1-4,16,共5页Shandong Agricultural Sciences

基　　金：山东省农业科学院青年科研基金项目(2016YQN33);山东省农业科学院农业科技创新工程项目(CXGC2018D06);山东省现代农业产业技术体系驴产业体系(SDAIT-27-10)

摘　　要：组蛋白乙酰基转移酶上调表达与抗逆性有一定相关性。集胞藻Synechocystis sp.PCC 6803 Slr1501是一个未知蛋白,预测其属于组蛋白乙酰基转移酶GNAT家族N-乙酰基转移酶。为了进一步明确slr1501基因功能,本研究从集胞藻6803中克隆slr1501基因,成功构建了pET-28a(+)-slr1501原核表达载体,并转化至大肠杆菌BL21(DE3)进行分析。经1 mmol/L IPTG诱导2 h后Slr1501蛋白成功表达,表达量随诱导时间的延长而增加,且主要以包涵体形式表达。Western blot检测结果显示Slr1501重组蛋白特异性表达。本试验结果为进一步研究该基因的功能奠定了基础。The up-regulated expression of histone acetyltransferase was correlated with the stress resistance. Slr1501 of Synechocystis sp. PCC 6803 was an unknown protein, and was predicted to be the member of GNAT family of histone acetyltransferases. In this study, slr1501 was amplified with the template of Synechocystis sp. PCC 6803 genomic DNA and ligased with pET-28a(+) by Nde Ⅰ and Xho Ⅰ enzyme sites. pET- 28a(+)- slr1501 expression plasmid was constructed and transformed into E.coli BL21 (DE3), which was successfully expressed after 1 mmol/L IPTG induction for two hours. The expression level of Slr1501 increased with the extension of induction time, and was mainly expressed in inclusion body form. The expression of Slr1501 in E.coli laid a foundation for further study on the function of the gene.

关 键 词：集胞藻6803 乙酰基转移酶 slr1501 原核表达 基因功能 

分 类 号：S555.6[农业科学—作物学] Q785[生物学—分子生物学]