CRMP3在青少年皮质发育障碍型癫痫脑组织中的表达研究  

Study on the expression of CRMP3 in brain tissues of adolescents with epilepsy induced by cortical developmental disorder

作　　者：覃璐 余瑞杭 蒋艳[1] QIN Lu;YU Ruihang;JIANG Yan(Department of Neurology, Zhejiang Provincial Hospital of TCM, Hangzhou 310006, China;Department of Emergency, Zhejiang Provincial Hospital of TCM, Hangzhou 310006, China)

机构地区：[1]浙江省中医院神经内科,浙江杭州310006 [2]浙江省中医院急诊科,浙江杭州310006

出　　处：《中国现代医生》2019年第19期18-21,169,共4页China Modern Doctor

基　　金：浙江省自然科学基金一般项目(LY19H090001)

摘　　要：目的探讨CRMP3在青少年皮质发育障碍型癫痫脑组织中的表达情况.方法将收集的青少年皮质发育障碍型癫痫脑组织及脑外伤术后正常脑组织标本分为皮质发育障碍组(MCD组,n=15)和对照组(control组,n=11).采用实时定量PCR方法检测CRMP3基因表达情况,采用Western-blot及免疫组化方法检测两组之间CRMP3的蛋白质表达水平.结果实时定量PCR结果提示CRMP3的mRNA表达水平在MCD组中表达增高,差异有统计学意义(P<0.05).Western-blot及免疫组化结果提示在MCD组中CRMP3蛋白表达水平较control组显著上调,且CRMP3主要表达于神经元细胞质中(P<0.05).结论 CRMP3在青少年皮质发育障碍型癫痫脑组织中高表达,可能与皮质发育障碍型癫痫的发生发展具有相关性.Objective To investigate the expression of CRMP3 in brain tissues of adolescents with epilepsy induced by cortical developmental disorder. Methods The collected brain tissues of adolescents with epilepsy induced by cortical developmental disorder and normal specimens of brain tissue after the surgery of brain trauma were divided into cortical developmental disorder group (MCD group, n=15) and control group (control group, n=11). Real-time quantitative PCR was used to detect the expression of CRMP3 gene. The protein expression level of CRMP3 between the two groups was detected by Western-blot and immunohistochemistry. Results According to the real-time quantitative PCR results, the expression of CRMP3 gene was increased in the MCD group, and the difference was statistically significant(P<0.05). According to the Western-blot and immunohistochemistry results, the expression level of CRMP3 protein was significantly up-regulated in the MCD group compared with the control group, and CRMP3 was mainly expressed in neuronal cytoplasm(P<0.05). Conclusion CRMP3 is highly expressed in the brain tissues of adolescents with epilepsy induced by cortical developmental disorder, which may be related to the occurrence and development of epilepsy induced by cortical developmental disorder.

关 键 词：皮质发育障碍 癫痫 CRMP3 青少年 

分 类 号：R742.1[医药卫生—神经病学与精神病学]