刺槐Genomic-SSR与EST-SSR遗传差异性分析  

Genetic Differences of Black Locust(Robinia pseudoacacia) with Genomic-SSR and EST-SSR Markers

作　　者：董黎[1] 张江涛[2] 文彦忠 牛东升 田书勇 孙宇涵[1] 李云[1] Dong Li;Zhang Jiangtao;Wen Yanzhong;Niu Dongsheng;Tian Shuyong;Sun Yuhan;Li Yun(National Engineering Laboratory for Tree Breeding/ College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083;Henan Academy of Forestry, Zhengzhou 450008;Propagation and Breeding Farm for Improved Forest Trees of Jixian County, Jixian Shanxi 042200;National Nursery of Guanxian County, Guanxian Shandong 252500)

机构地区：[1]林木育种国家工程实验室/北京林业大学生物科学与技术学院,北京100083 [2]河南省林业科学研究院,郑州450008 [3]吉县林木良种繁育场,山西吉县042200 [4]国有冠县苗圃,山东冠县252500

出　　处：《中国农学通报》2019年第19期49-57,共9页Chinese Agricultural Science Bulletin

基　　金：北京林业大学重大科研成果培育项目“刺槐种质资源评价、品种选育与产业化应用”(2017CGP007);林业公益性行业科研专项“刺槐属种质资源收集保存与创新利用研究”(201304116);国家林业局科技发展中心项目“北林槐1号新品种转化应用试点”(XPC-2015-3);国家林业局科技推广项目“四倍体刺槐扦插繁殖方法推广示范”([2015]53号);中央高校基本科研业务费专项资金资助“重要生态抗逆树种育种与高效繁殖技术”(2016BLPX13);国家自然科学基金项目“刺槐不去雄条件下实施控制授粉的花粉竞争与子代选择机制”(31570677)

摘　　要：为在刺槐育种研究中合理选用不同来源的SSR分子标记,选取12个刺槐个体,试剂盒提取DNA后分别利用9对Genomic-SSR引物和9对EST-SSR引物进行扩增,并采取毛细管电泳检测扩增产物。利用所得条带信息及相关软件对2种SSR分子标记进行多态性、遗传相似系数相关性以及聚类等方面的比较分析。结果显示,刺槐Genomic-SSR平均检测到的条带数为6、Shannon多样性指数为1.3833、观测杂合度为0.5749、期望杂合度为0.6832;EST-SSR平均检测到的条带数为5.1、Shannon多样性指数为1.2711、观测杂合度为0.5648、期望杂合度为0.6526。由Genomic-SSR计算得到的个体间的遗传相似系数以及聚类结果与2种SSR标记综合计算得到的遗传相似系数和聚类结果更为相似。结果表明,刺槐Genomic-SSR与EST-SSR存在一定的遗传差异性,但差异并不显著;刺槐Genomic-SSR能更加准确地揭示基因型之间的遗传关系;刺槐EST-SSR具有相对较强的保守性。The study aims at selecting the SSR molecular markers reasonably in the breeding of black locust.12 individuals of black locust were used as the materials, the total genomic DNA was extracted by kit. 9 Genomic-SSR and 9 EST-SSR primers were used for PCR amplification and the products were separated by capillary electrophoresis. Comparative analysis of polymorphism, correlation of genetic similarity coefficients and cluster analysis between Genomic-SSR and EST-SSR were conduct by related software according to the information of the electrophoretic bands. The average number of bands detected by Genomic-SSR was 6.0,Shannon index was 1.3833, observed heterozygosity was 0.5749 and expected heterozygosity was 0.6832. The average number of bands detected by EST-SSR was 5.1, Shannon index was 1.2711, observed heterozygosity was 0.5648 and expected heterozygosity was 0.6526. Genetic similarity coefficients and results of cluster analysis between individuals calculated from Genomic-SSR were more similar to the comprehensive results calculated from the two kinds of SSR markers. There are genetic differences between Genomic-SSR and ESTSSR of black locust, but the differences are not significant. Genomic-SSR of black locust can more accurately reflect genetic relationship of different genotypes. EST-SSR of black locust is relatively more conservative.

关 键 词：刺槐 GENOMIC-SSR EST-SSR 遗传相似系数 遗传差异性 

分 类 号：S792.97[农业科学—林木遗传育种] Q789[农业科学—林学]