空肠弯曲菌的侧向流RPA快速检测方法研究  

Rapid Detection of Campylobacter Enterococci by Lateral Flow RPA

作　　者：刘小青[1] 王远洋 陈佳平 黄静敏[1] 肖承荣 陈血建[1] 陈晶[1] Liu Xiaoqing;Wang Yuanyang;Chen Jiaping;Huang Jingmin;Xiao Chengrong;Chen Xuejian;Chen Jing(Shenzhen Academy of Metrology & Quality inspection,Shenzhen 518131,China)

机构地区：[1]深圳市计量质量检测研究院

出　　处：《广东化工》2019年第14期247-248,253,共3页Guangdong Chemical Industry

摘　　要：目的:建立一种可现场快速检测空肠弯曲杆菌的方法。方法:应用重组酶聚合酶等温扩增(recombinase polymerase amplification,RPA)技术,设计引物探针,建立侧向流RPA法,使用特异性、灵敏性实验对该方法进行验证。结果:实验所用的所有非空肠弯曲菌在侧向流试剂盒上都是阴性的,空肠弯曲菌都是阳性的;该方法的检出限为1.2×10^3 CFU/mL。结论:本研究制作的RPA技术制作的侧向流试剂盒特异性和灵敏性良好,简单快捷,具有较强的应用价值。Objective:To establish a rapid method for the detection of Campylobacter jejuni in situ.Methods:Using recombinase polymerase amplification(RPA)technique,primer probes were designed,and Lateral Flow RPA Method was established.Specificity and sensitivity tests were used to verify the method.Result:All non-campylobacter jejuni used in the experiment were negative on the side-flow kit,and Campylobacter jejuni was positive.The detection limit of this method was 1.2×10^3 CFU/mL.Conclusion:The lateral flow kit made by RPA technology in this study has good specificity and sensitivity,simplicity and rapidity,and owns strong application value.

关 键 词：空肠弯曲菌 重组酶聚合酶等温扩增技术 侧向流RPA 

分 类 号：TQ[化学工程]