Effect of compound Yindan decoction on alpha-naphthylisothiocyanate-Induced acute intrahepatic cholestasis in rats  

作　　者：Sun Fengxia Li Xiaoling Xu Chunjun Sui Jingli Li Pan Wu Jingjing 

机构地区：[1]Department of Infections, Beijing Hospital of Traditional Chinese Medicine, Affiliated to the Capital Medical University, No.23, Back Road of the Art Gallery, Dongcheng District, Beijing 100010, China [2]Infections Department, Beijing Hospital of Traditional Chinese Medicine Shunyi Branch, Beijing 101300,China

出　　处：《Journal of Traditional Chinese Medicine》2019年第3期315-323,共9页中医杂志（英文版）

基　　金：Supported by National Natural Science Foundation of China:Research on the mechanism of clearing away damp and heat and cooling and activating blood method based on the transcriptional regulation signaling pathway of hepatic membrane transport protein for the treatment of acute intrahepatic cholestasis(No.81573897)

摘　　要：OBJECTIVE:To investigate the therapeutic mechanism of compound Yindan decoction (CYD) in a rat model of acute intrahepatic cholestatic (AIC).METHODS:A total of 108 adult male rats were randomly divided into control (n =18) and AIC groups (n =90).AIC was induced in rats using alpha-naphthylisothiocyanate (ANIT)(75 mg/kg,10 mL/kg in corn oil,p.o.).Then,90 AIC rats were randomly divided into five groups:a control group (n =18),a CYD high dose group (n =18),a CYD middle dose group (n =18),a CYD low dose group (n =18),and a ursodeoxycholicacid (UDCA) group (n =18).According to sampling time,each group was subdivided into three subgroups:24 h (n =6),48 h (n =6),and 72 h groups (n =6).The CYD-high,-middle and-low groups were orally administered 24.48,12.24,and 6.12 g.kg-1.d-1 modified CYD,respectively,while the model group was given 20 mL/kg of body weight of distilled water once a day.The UDCA group was given 67.5 mg.kg-1.d-1 UDCA once a day.Radioimmunity assay was used to detect the activity of alanine aminotransferase (ALT),aspartate aminotransferase (AST),alkaline phosphatase (ALP),gamma-glutamyl transpeptidase (GGT) and the levels of total bilirubin (TBil) and indirectbiliruin (DBil) in rats.Reverse transcription quantitative polymerase chain reaction (qRT-PCR),Western blot analysis,and immunohistochemistry were used to detect multidrug resistance-associated protein 2 (MRP2) expression.In vitro,HepG2 hepatocellular carcinoma cells were treated with CYD medicated serum at a concentration of 15 mol/L.MRP2 and retinoid X receptor alpha (RXRα) expression was analyzed by qRT-PCR and Western blotting.RESULTS:Serum levels of ALT,AST,GGT,ALP,TBil,and DBil were significantly reduced in the CYD and positive drug groups compared with the control group (P < 0.05 and P < 0.01,respectively).Pathological changes in rat liver tissues at 72 h in the CYD-high and-medium dose groups and positive drug group were not significant compared with the control group.CYD and UDCA treatment ameliorated ANIT-induced biliary epithelial cell proOBJECTIVE:To investigate the therapeutic mechanism of compound Yindan decoction（CYD）in a rat model of acute intrahepatic cholestatic（AIC）.METHODS:A total of 108 adult male rats were randomly divided into control（n= 18）and AIC groups（n=90）.AIC was induced in rats using alpha-naphthylisothiocyanate（ANIT）（75 mg/kg,10 mL/kg in corn oil,p.o.）.Then,90 AIC rats were randomly divided into five groups:a control group（n = 18）,a CYD high dose group（n = 18）,a CYD middle dose group（n= 18）,a CYD low dose group（n= 18）,and a ursodeoxycholic acid（UDCA）group（n = 18）.According to sampling time,each group was subdivided into three subgroups:24 h（n=6）,48 h（n=6）,and 72 h groups（n = 6）.The CYD-high,-middle and-low groups were orally administered 24.48,12.24,and 6.12 g·kg-1·d-1 modified CYD,respectively,while the model group was given 20 mL/kg of body weight of distilled water once a day.The UDCA group was given 67.5 mg·kg-1·d-1 UDCA once a day.Radioimmunity assay was used to detect the activity of alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,alkaline phosphatase（ALP）,gamma-glutamyl transpeptidase（GGT）and the levels of total bilirubin（TBil）and indirect biliruin（DBil）in rats.Reverse transcription quantitative polymerase chain reaction（qRT-PCR）,Western blot analysis,and immunohistochemistry were used to detect multidrug resistance-associated protein 2（MRP2）expression.In vitro,HepG2 hepatocellular carcinoma cells were treated with CYD medicated serum at a concentration of 15 mol/L.MRP2 and retinoid X receptor alpha（RXRa）expression was analyzed by qRT-PCR and Western blotting.RESULTS:Serum levels of ALT,AST,GGT,ALP,TBil,and DBil were significantly reduced in the CYD and positive drug groups compared with the control group（P<0.05 and P<0.01,respectively）.Pathological changes in rat liver tissues at 72 h in the CYD-high and-medium dose groups and positive drug group were not significant compared with the control group.CYD a

关 键 词：Cholestasis intrahepatic MULTIDRUG resistance-associated proteins RETINOID X receptor alpha COMPOUND Yindan DECOCTION 

分 类 号：R[医药卫生]