应用益生菌监测牛奶抗生素残留  

Screening of multiantibiotic residues in milk by using probiotics

作　　者：王温琪 唐志德 周燕[1] 张浩[1] 薛娇 杜季梅[1] WANG Wenqi;TANG Zhide;ZHOU Yan;ZHANG Hao;XUE Jiao;DU Jimei(School of Laboratory Medicine,Wenzhou Medical University,Wenzhou,Zhejiang 325035,China)

机构地区：[1]温州医科大学检验医学院,温州医科大学微生态研究所,浙江温州325035 [2]湖北宜昌市中心医院检验科,湖北宜昌443000

出　　处：《中国微生态学杂志》2019年第7期787-792,共6页Chinese Journal of Microecology

基　　金：浙江省科技厅公益项目(2013C32070;LGF18H180012)

摘　　要：目的探索肠道益生菌在抗生素残留监测中的应用并建立牛奶中多种抗生素残留的微生物学筛检方法。方法厌氧培养分离健康人肠道益生菌并用纸片扩散法检测其对四环素、红霉素、青霉素、庆大霉素、氯霉素和万古霉素的敏感性。根据药敏结果及产酸能力选择工作菌株。建立基于厌氧培养的益生菌抗生素残留筛检方法并优化实验条件,测定其对不同抗生素的检测限,通过测定加标模拟阳性和阴性样本计算方法的准确性。结果短双歧杆菌A2可用于牛奶抗生素残留筛检。应用短双歧杆菌筛检牛奶中四环素、红霉素和青霉素的检测限分别为80μg/L、40μg/L和3μg/L,检测加标四环素、红霉素和青霉素的牛奶样品准确性为100.0%、92.5%和90.0%,无假阴性结果。结论应用益生菌的筛检方法敏感、简便,对环境和操作者友好,适用于监测牛奶中四环素、红霉素和青霉素等多种抗生素。Objective To explore the feasibility of probiotics as working strain in screening antibiotic residues and develop a microbial method for screening of multi-antibiotic residuals in milk.Methods Strains of intestinal probiotics were isolated from healthy young men and their susceptibilities to Tetracycline,Erythromycin,Ampicillin,Gentamycin,Chloramphenicol and Vancomycin were measured by using Kirby-Bauer method.The working strain was selected based on its high sensitivities to antibiotics and good acid-producing capacity in culture media.Based on anaerobic culture,the screening method was established and optimized.The detection limit to each antibiotic was determined under the optimized condition,and the accuracies of calculations for standard(positive)and negative spiked simulated samples were determined.Results The strain of Bifidobacterium breve A2 was an ideal working strain for screening of antibiotic residues.The detection limit of the method for Tetracycline,Erythromycin and Ampicillin was 90μg/L,40μg/L and 3μg/L respectively,while the accuracy was 100.0%,92.5%and 90.0%respectively,with no false negative results.Conclusion The method using Bifidobacterium breve A2 was sensitive,simple,eco-friendly and suitable for screening of Tetracycline,Erythromycin and Ampicillin residues in milk.

关 键 词：益生菌 抗生素残留 牛奶 

分 类 号：R155.5[医药卫生—营养与食品卫生学]