酶法催化漆蜡合成漆蜡基异丙酯工艺研究  

Lipase catalyzed synthesis of isopropyl ester from lacquer wax

作　　者：马余璐 王成章[1,2,3] 周昊 叶建中[1,2,3] 胡军 MA Yulu;WANG Chengzhang;ZHOU Hao;YE Jianzhong;HU Jun(Institute of New Technology of Forestry,CAF,Beijing 100091,China;Key and Open Lab. of Forest Chemical Engineering, SFA, National Engineering Lab. for Biomass Chemical Utilization, Institute of Chemical Industry of Forest Products, CAF, Nanjing 210042, China;Nanjing Zhongsen Biological Technology Co.,Ltd., Nanjing 210000,China;Hubei Zixin Biological Technology Co., Ltd., Wuhan 435666,China)

机构地区：[1]中国林业科学研究院林业新技术研究所,北京100091 [2]中国林业科学研究院林产化学工业研究所,生物质化学利用国家工程实验室,国家林业局林产化学工程重点开放性实验室,南京210042 [3]南京中森生物科技有限公司,南京210000 [4]湖北紫鑫生物科技有限公司,武汉435666

出　　处：《中国油脂》2019年第7期48-52,共5页China Oils and Fats

基　　金：中国林业科学研究院林业新技术研究所中央级公益性科研院所基本科研业务费专项资金(CAFINT2015K01)

摘　　要：以脂肪酶为催化剂,催化漆蜡与异丙醇进行酯交换反应合成漆蜡基异丙酯。首先采用傅里叶红外光谱对合成产物进行结构表征,然后采用单因素试验和正交试验对酯交换反应条件进行了优化,并对最佳条件下合成的漆蜡基异丙酯的组成进行了GC-MS分析。结果表明:在脂肪酶催化下,漆蜡可与异丙醇反应生成目标产物;Novozyme 435脂肪酶催化酯交换反应的最佳工艺条件为醇蜡比4∶1、脂肪酶加量10 g/L、反应温度55℃、反应时间36 h,在此条件下酯交换率为86. 7%,漆蜡基异丙酯的酸价(KOH)为3. 28 mg/g;漆蜡基异丙酯主要由棕榈酸异丙酯(73. 64%)、油酸异丙酯(19. 39%)、硬脂酸异丙酯(6. 15%)和亚油酸异丙酯(0. 82%)组成。Synthesis of isopropyl ester from lacquer wax by transesterification with lipase catalyst was studied. The structure of the synthesized product was characterized by FT-IR. The transesterification conditions were optimized by single factor experiment and orthogonal experiment,and the compositions of the product synthesized under the optimal conditions were analyzed by GC-MS. The results showed that the isopropyl ester could be obtained by transesterification of lacquer wax and isopropanol with lipase as catalyst. The optimal conditions were obtained as follows: ratio of isopropanol to lacquer wax 4∶ 1,Novozymes 435 lipase dosage 10 g/L,reaction temperature 55 ℃,reaction time 36 h. Under these conditions,the transesterification rate was 86. 7%,and the acid value of isopropyl ester from lacquer wax was 3. 28 mgKOH/g. The product was mainly composed of isopropyl palmitate (73. 64%), isopropyl oleate (19. 39%),isopropyl stearate (6. 15%) and isopropyl linoleate (0. 82%).

关 键 词：漆蜡 酶催化 漆蜡基异丙酯 

分 类 号：TQ032[化学工程] O643.32[理学—物理化学]