酒酒球菌(Oenococcus oeni)产胞外聚合物培养基的优化及其抗冷冻干燥性能评价  

Culture medium optimization for Oenococcus oeni producing extracellular polymeric substances and their cryoprotective ability

作　　者：王继锋 石侃 安玮 余东亮[1,4] 刘树文 何玲[5] WANG Jifeng;SHI Kan;AN Wei;YU Dongliang;LIU Shuwen;HE Ling(College of Enology, Northwest A&F University, Yangling 712100, China;Shaanxi Engineering Research Center for Viti-Viniculture, Yangling 712100, China;Heyang Experimental and Demonstrational Stations for Grape, Weinan 715300, China;Chateau Kings International Co., Ltd. Qinhuangdao, Changli 066600, China;College of Horticulture, Northwest A&F University, Yangling 712100, China)

机构地区：[1]西北农林科技大学葡萄酒学院,陕西杨凌712100 [2]陕西省葡萄与葡萄酒工程技术研究中心,陕西杨凌712100 [3]陕西省合阳葡萄试验示范站,陕西渭南715300 [4]秦皇岛金士国际葡萄酒庄有限公司,河北昌黎066600 [5]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《食品与发酵工业》2019年第15期157-163,共7页Food and Fermentation Industries

基　　金：贺兰山东麓特色葡萄酒生产工艺体系及葡萄酒标准体系建立研究(2016BZ0603);“十三五”国家科技支撑计划项目(2016YFD0400504-01);国家现代农业产业技术体系建设专项(nycytx-30-ch-03)

摘　　要：为了研究酒酒球菌( Oenococcus oeni )产胞外聚合物(extracellular polymeric substances, EPS)在冷冻干燥过程中的保护性能,通过优化培养基提高EPS产量,并通过EPS对细菌冷冻干燥存活率的影响以及观察冷冻干燥过程中酒酒球菌内部及外表面的变化,评价其抗冷冻干燥的性能。正交试验优化可得,在初始pH值4.8、蛋白胨含量20 g/L、葡萄糖含量15 g/L的条件下,能产119.7 mg/100 mL酒酒球菌EPS。EPS作为冷冻干燥保护剂时细菌冷冻干燥后的存活率为70.97%,高于其他常规保护剂,扫描电子显微镜(scanning electron microscope,SEM)和透射电子显微镜(transmission electron microscope,TEM)的图像表明添加冷冻干燥保护剂的实验组细胞形态更加完整,说明EPS有明显的冷冻干燥保护作用。该研究初步探究了酒酒球菌EPS冷冻干燥保护的机制,并为EPS在冷冻干燥保护剂中的应用提供理论基础。This study aimed to optimize the culture medium for producing extracellular polymeric substances (EPS) by Oenococcus oeni and evaluate the cryoprotective ability of EPS by scanning electron microscope (SEM), transmission electron microscope (TEM) and the survival rate analysis of O. oeni after freeze-drying. It was found that119.7 mg/100 mL EPS was produced under the optimized condition: an initial pH of 4.8 with20 g/L peptone and15 g/L glucose. The survival rate of bacteria after freeze-drying was 70.97% when EPS was used as a cryoprotectant, which was higher than those of other conventional cryoprotectants. Besides, EPS had obvious protective effects on freeze-dried bacteria, as the morphology of cells in experimental groups with cryoprotectants were more complete. This study prelimin.arily explored the mechanisms of EPS regarding protecting freeze-dried O. oeni , which provides a theoretical basis for applying EPS in developing freeze-drying protectants.

关 键 词：酒酒球菌 胞外聚合物 冷冻干燥 存活率 

分 类 号：TS261.1[轻工技术与工程—发酵工程]