PAX2 regulates NRP-1 expression in renal tubular epithelial cellsto promote epithelial-to-mesenchymal transition and renal fibrosis  

作　　者：Ling Hou Yue Du Chengguang Zhao Xiuli Wang Yubin Wu 

机构地区：[1]Department of pediatrics,Shengjing Hospital of China Medical University,Shenyang

出　　处：《Journal of Pediatric Diseases》2018年第1期32-40,共9页儿科疾病杂志

基　　金：grants from the Natural Science Foundation of Liaoning Province,China (2013225086,2013021099,2015020492);Science and Technology Planning Project of Shenyang City,China (F13-221- 9-59);Excellent Doctoral Project Fund of Shengjing Hospital of China Medical University (MC96).

摘　　要：To observe the expression of neuropilin-1 (NRP-1) induced by paired-box gene 2 (PAX2) during the process of epithelial-to-mesenchymal transition (EMT) and renal fibrosis in unilateral ureteral obstruction (UUO) model in rats, and explore the mechanism of EMT induced by PAX2. Methods: The recombinant lentivirus expression vector for PAX2 was constructed and transfected into rat normal renal tubular epithelial cell line (NRK52E). The experimental cells were divided into three groups: transfection group, empty vector group, and normal group. E-cadherin and oup, empty vector group, and normal and real-time PCR. Expression of NRP-1 was detected by western blot, real-time PCR, and immunofluorescence. Sixty male Wistar rats were randomly divided into two groups: the sham-operation group (n = 30) underwent left ureteral dissection, the UUO group (n = 30) underwent left ureteral ligation. Post-operation on days 3, 7, 14, 21 and 28, 6 rats from each of the groups were sacrificed and the obstructed kidneys were dissected out. The histopathological changes were observed by hematoxylin-eosin and Masson staining. E-cadherin and dhSMA were detected by western blot and immunohistochemistry. Expression of NRP-1 and PAX2 were determined by western blot, immunohistochemistry, and real-time PCR. Results: Expression of NRP-1 mRNA and protein and d by western blot, immunoh < 0.05) while E-cadherin protein expression decreased (P < 0.05) in the transfection group as compared to the empty vector group in vitro. In the UUO group, fibrosis was obvious, and there was decreased expression of E-cadherin protein (P < 0.05) and increased expression of 5) and increased expression in (Pious, and < 0.05) in comparison to the sham group. Conclusion: NRP-1 maybe mediate PAX2-induced EMT in renal tubular epithelial cells and renal fibrosis.

关 键 词：renal interstitial fibrosis ureteral OBSTRUCTION epithelial-to-mesenchymal transition paired-box gene 2 NEUROPILIN-1 

分 类 号：R[医药卫生]