Profiling and identification of aqueous extract of Cordyceps sinensis by ultra-high performance liquid chromatography tandem quadrupole-orbitrap mass spectrometry  

作　　者：YAO Chang-Liang QIAN Zheng-Ming TIAN Wen-Shuai XU Xiao-Qian YAN Yu SHEN Yao LU Song-Mao LI Wen-Jia GUO De-An 

机构地区：[1]R&D Department, GenChim Testing (Shanghai) Co., Ltd., Shanghai 200131, China [2]Key Laboratory of State Administration of Traditional Chinese Medicine, Sunshine Lake Pharma Co. Ltd., Guangdong 523850, China [3]Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China

出　　处：《Chinese Journal of Natural Medicines》2019年第8期631-640,共10页中国天然药物（英文版）

基　　金：supported by National Key R&D Program of China(Nos.2018YFC1707900 and 2018YFC1707300)

摘　　要：Characterization of aqueous extract in traditional Chinese medicine(TCM) is challenging due to the poor retention of the analytes on conventional C18 columns. This study presents a systematic characterization method based on a rapid chromatographic separation(8 min) on a polar-modified C18(Waters Cortecs T3) column of aqueous extract of Cordyceps sinensis. UHPLC-HRMS method was used to profile components in both untargeted and targeted manners by full MS/PIL/dd-MS2 acquisition approach. The components were identified or tentatively identified by reference standards comparison, fragmentation rules elucidation and available databases search. A total of 91 components, including 10 nucleobases, 20 nucleosides, 39 dipeptides, 18 amino acids and derivatives and 4 other components, were characterized from the aqueous extract of C. sinensis. And this was the first time to systematically report the presence of nucleosides and dipeptides in C. sinensis, especially for modified nucleosides. The chemical basis inquiry of this work would be beneficial to mechanism exploration and quality control of C. sinensis and related products. Meanwhile, this work also provided an effective solution for characterization of aqueous extract in TCM.Characterization of aqueous extract in traditional Chinese medicine(TCM) is challenging due to the poor retention of the analytes on conventional C18 columns. This study presents a systematic characterization method based on a rapid chromatographic separation(8 min) on a polar-modified C18(Waters Cortecs T3) column of aqueous extract of Cordyceps sinensis. UHPLC-HRMS method was used to profile components in both untargeted and targeted manners by full MS/PIL/dd-MS2 acquisition approach. The components were identified or tentatively identified by reference standards comparison, fragmentation rules elucidation and available databases search. A total of 91 components, including 10 nucleobases, 20 nucleosides, 39 dipeptides, 18 amino acids and derivatives and 4 other components, were characterized from the aqueous extract of C. sinensis. And this was the first time to systematically report the presence of nucleosides and dipeptides in C. sinensis, especially for modified nucleosides. The chemical basis inquiry of this work would be beneficial to mechanism exploration and quality control of C. sinensis and related products. Meanwhile, this work also provided an effective solution for characterization of aqueous extract in TCM.

关 键 词：AQUEOUS extract CORDYCEPS SINENSIS DIPEPTIDES NUCLEOSIDES 

分 类 号：R917[医药卫生—药物分析学]