利用超临界CO2流体萃取小球藻精油中活性成分的工艺  

Extraction of Active Components from Chlorella Essential Oil by Supercritical CO2 Fluid

作　　者：陈金明 张文焕 罗苏芹 戴宏杰 黄惠华[3] CHEN Jinming;ZHANG Wenhuan;LUO Suqin;DAI Hongjie;HUANG Huihua(Guangzhou Levon Flavour & Fragrance Technology Co., Ltd. Guangzhou 510663;Cadbury Confectionery (Guangzhou) Co., Ltd. Guangzhou 510730;School of Food Science and Engineering, South China University of Technology Guangzhou 510640)

机构地区：[1]广州联丰香料科技有限公司,广州510663 [2]吉百利糖果(广州)有限公司,广州510730 [3]华南理工大学食品科学与学院,广州510640

出　　处：《食品工业》2019年第7期10-15,共6页The Food Industry

基　　金：广州市科技计划项目(2010Z1-E221)

摘　　要：采用超临界CO2萃取小球藻精油,通过单因素试验研究了萃取温度、萃取压力、萃取时间和夹带剂用量对精油得率和精油中叶黄素浓度的影响,进一步采用响应面优化萃取工艺,得到小球藻精油的最佳萃取工艺是:萃取压力为24 MPa,萃取温度为36℃,萃取时间为3 h,夹带剂无水乙醇用量为155 mL。此时小球藻精油得率为5.68±0.06 (g/100 g藻粉)。叶黄素的最佳萃取工艺是:萃取压力为16 MPa,萃取温度为30℃,萃取时间为2.8 h,夹带剂无水乙醇用量为199 mL。得到叶黄素浓度为7.13±0.10 (mg/g小球藻精油)。此研究为超临界CO2萃取小球藻精油及其活性成分提供基础数据。Supercritical CO2 was used to extract the essential oil of chlorella. The effects of extraction temperature, pressure,time and entrainer dosage on the yield of essential oil and the concentration of lutein in the essential oil were studied by single factor experiment. The extraction process of chlorella essential oil was then optimized by using response surface optimization.The extraction conditions were optimized as 24 MPa of pressure, 36 ℃ of temperature, and 3 h of time and 155 mL of ethanol content of entrainer. The yield of Chlorella oil was 5.68±0.06 g/100 g powder. The extraction conditions for xanthophylls were optimized as 16 MPa of extraction pressure, 30 ℃ of temperature, 2.8 h of time, and 199 mL of ethanol, by which the concentration of xanthophylls reached to 7.13±0.10 mg/g chlorella oil.

关 键 词：小球藻 超临界CO2萃取 精油 叶黄素 响应面 

分 类 号：TQ914.2[化学工程]