卵运铁蛋白消化稳定性及消化产物致敏性分析  

The digestibility of ovotransferrin and the allergenicity of the digested ovotransferrin products

作　　者：李慧慧[1,2] 佟平[1] 武涌[1,3] 陈红兵[1,3] 高金燕[1,2] LI Huihui;TONG Ping;WU Yong;CHEN Hongbing;GAO Jinyan(State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047;School of Food Science & Technology,Nanchang University,Nanchang 330047;Sino-German Joint Research Institute,Nanchang University,Nanchang 330047)

机构地区：[1]南昌大学食品科学与技术国家重点实验室,南昌330047 [2]南昌大学食品学院,南昌330047 [3]南昌大学中德联合研究院,南昌330047

出　　处：《食品科技》2019年第7期67-74,共8页Food Science and Technology

基　　金：国家自然科学基金项目(31660436);国家重点研发计划项目(2018YFD0400304)

摘　　要：为了评价卵运铁蛋白消化稳定性及消化产物的致敏性,采用体外静态模拟婴幼儿消化模型对卵运铁蛋白进行消化,利用SDS-PAGE、RP-HPLC和质谱对卵运铁蛋白消化稳定性进行表征,通过ELISA检测消化产物的特异性IgE结合能力,利用KU812细胞脱颗粒模型评估消化产物的致敏性。结果显示,鸡蛋卵运铁蛋白经过模拟胃液消化后,大部分被消化,产生的肽段分子质量主要在3~12ku,经过模拟小肠液进一步被消化,再经过BBM酶的作用,卵运铁蛋白全部降解成小片段;卵运铁蛋白及其经胃液、胃-小肠、胃-小肠-BBM酶的消化产物与IgE特异性结合的OD值、细胞释放的β-氨基己糖苷酶、组胺、TNF-α、IL-4含量分别为1.032、0.679、0.389、0.328,19.90%、14.04%、10.95%、8.31%,665.13、446.62、374.24、359.67ng/mL,15.07、8.96、2.90、2.07pg/mL,5.97、2.04、0.95、0.82pg/mL。卵运铁蛋白可以被模拟婴幼儿消化液所消化,具有一定稳定性和抗消化能力,但仍保留较强的致敏性。In order to assess the digestibility of ovotransferrin and the allergenicity of the digested ovotransferrin,the digestibility was tested in the simulating digestion fluids of infants,followed by characterizing by SDS-PAGE,RP-HPLC and mass spectrometry.Moreover,the specific IgE binding capacity of the digested ovotransferrin was tested by ELISA,and the allergenicity was assessed in human basophil KU812 degranulation model.The results showed that most of ovotransferrin was digested by simulated gastric juice,and the produced peptides were mainly among 3~12 ku.Futhermore,ovotransferrin was further digested by gastrointestinal juice,and it was degraded into small fractions completely by BBM enzyme.The OD of specific IgE,the contents of releasedβ-HEX,histamine,TNF-α,IL-4 in ovotransferrin group and the digested ovotransferrin groups by simulated gastric juice,gastrointestinal juice and BBM enzyme,respectively,were 1.032,0.679,0.389,0.328;19.90%,14.04%,10.95%,8.31%;665.13,446.62,374.24,359.67 ng/mL;15.07,8.96,2.90,2.07 pg/mL;5.97,2.04,0.95,0.82 pg/mL,respectively.Ovotransferrin could be digested in the simulated infant digestion fluids and keep stability and resistance to the digestion to some extent.However,the relatively strong allergenicity of the digested ovotransferrin still existed.

关 键 词：卵运铁蛋白 消化 稳定性 致敏性 

分 类 号：TS253.4[轻工技术与工程—农产品加工及贮藏工程]