Performance comparison of chlorinated chiral stationary phases in supercritical fluid chromatography for separation of selected pyrrolidone derivatives  

作　　者：Anca-Elena Dascalu Alina Ghinet Muriel Billamboz Emmanuelle Lipka 

机构地区：[1]Univ. Lille, Inserm, U995 - LIRIC - Lille Inflammation Research International Center, F-59000 Lille, France [2]Ecole des Hautes Etudes d'Ingenieur (HEI), Laboratoire de Pharmacochimie, 13 rue de Toul, F-59046 Lille, France [3]UFR Pharmacie, Laboratoire de Chimie Analytique, BP 83, F-59006 Lille, France [4]'Alexandru Ioan Cuza’ University of Iasi, Faculty of Chemistry, Bd. Carol I nr. 11, 700506 Iasi, Romania

出　　处：《Journal of Pharmaceutical Analysis》2019年第4期248-253,共6页药物分析学报（英文版）

基　　金：supported by the State as part of the "Programme d’Investissement d’Avenir",under reference ANR10-IEED-0004-01

摘　　要：The effects of two chlorinated chiral stationary phases, namely, Lux Cellulose-2 and Lux i-Cellulose-5, flow-rate, percentage of co-solvent and chemical structures of the compounds on retention and resolution were studied within this article. In this work a backpressure of 150 bar, a temperature of 40 ℃ and 10% of methanol as co-solvent were chosen as operating conditions. The optimum flow-rate was 2 mL/min. The percentage of co-solvent was studied between 7.5% and 15%.We have observed that 15% of methanol gave the best results for most of the compounds. For all the derivatives, the Lux Cellulose-2 provided better resolutions going from 1.50 to 3.59 compared with Lux i-Cellulose-5.The effects of two chlorinated chiral stationary phases,namely,Lux Cellulose-2 and Lux i-Cellulose-5,flow-rate,percentage of co-solvent and chemical structures of the compounds on retention and resolution were studied within this article.In this work a backpressure of 150 bar,a temperature of 40 ℃ and10% of methanol as co-solvent were chosen as operating conditions.The optimum flow-rate was2 mL/min.The percentage of co-solvent was studied between 7.5% and 15%.We have observed that 15% of methanol gave the best results for most of the compounds.For all the derivatives,the Lux Cellulose-2 provided better resolutions going from 1.50 to 3.59 compared with Lux i-Cellulose-5.

关 键 词：ANTIBACTERIAL compounds CHIRALITY ENANTIOMERS FLOW-RATE Particle size 

分 类 号：R[医药卫生]