虎杖白藜芦醇、槲皮素异构体联用抗生素对耐药金黄色葡萄球菌的抑制作用  

Inhibition of Drug-resistant Staphylococcus aureus by Antibiotics Combined with Resveratrol and Quercetin Isomers

作　　者：陈尚岳 纪亚明 李玉环 于海霞 谢敬亮 舒挥文 孙颖玲 张妙良 薛喜才 何羽 CHEN Shang-yue;JI Ya-ming;LI Yu-huan;YU Hai-xia;XIE Jing-liang;SHU Hui-wen;SUN Ying-ling;ZHANG Miao-liang;XUE Xi-cai;HE Yu(Beijing Xiao Tang Shan Hospital, Beijing 102211, China)

机构地区：[1]北京小汤山医院

出　　处：《食品工业科技》2019年第16期97-101,共5页Science and Technology of Food Industry

基　　金：国家自然科学基金项目(81603627)

摘　　要：为研究药物联用对耐药性金黄色葡萄球菌(MRSA)的抑制作用,本文将虎杖白藜芦醇(RES)、槲皮素异构体(MOR)与抗生素(红霉素、苯唑西林和环丙沙星)进行联合用药,分别进行了抑菌实验、杀菌曲线和细胞毒性试验研究。结果表明,RES或MOR与红霉素联用时均不能增强对MRSA的抑制作用,表现为无关或药物拮抗效应;而RES或MOR与苯唑西林、环丙沙星联用时,对MRSA的抑制作用均显著增强(p <0.05),抗生素用量分别减少了50%和75%,其中最佳抑菌组合为MOR联用环丙沙星(750 mg/L+25 mg/L),抑菌指数(FIC)为0.5,表现为药物协同作用。细胞毒性实验结果表明,RES联用抗生素不会增加药物的细胞毒性,而MOR联用苯唑西林或环丙沙星则呈现微弱细胞毒性增加,细胞存活率下降7.2%~8.9%。因此,RES、MOR可作为苯唑西林和环丙沙星的抗菌增强剂。In order to explore the inhibitory effect of combination therapy on methicillin-resistant Staphylococcus aureus( MRSA),polygonum cuspidatum resveratrol and quercetin isomer were combined with antibiotics( erythromycin,oxacillin and ciprofloxacin) in this work,the antibacterial experiment,bactericidal curve and cytotoxicity test were carried out.Results showed that erythromycin combined with polygonum cuspidatum resveratrol or quercetin isomers could not enhance the antibacterial activity,and even showed the irrelevant or drug antagonistic effects. However,resveratrol or quercetin isomers associated with oxacillin or ciprofloxacin could enhance the antibacterial effect of antibiotics on MRSA,and respectively reduce the dosage of antibiotics by 50% and 75%.The best antimicrobial combination was quercetin isomer combined with ciprofloxacin( 750 mg/L+ 25 mg/L),the fractional inhibitory concentration index( FIC) was 0.5,which suggested that it was a synergistic effect.However,the oxacillin or ciprofloxacin associated with quercetin isomer,which compared with the antibiotics alone,showing the weak cytotoxicity to mouse cells,cell viability was decreased by 7.2%~8.9%,while it was non-toxic increased to the oxacillin or ciprofloxacin combined with resveratrol.Therefore,polygonum cuspidatum resveratrol and quercetin isomers could be used as antibiotic enhancers of oxacillin or ciprofloxacin.

关 键 词：耐药性金黄色葡萄球菌(MRSA) 抗生素 联合用药 抑制作用 

分 类 号：TS201.3[轻工技术与工程—食品科学]