代谢组学技术在兽药违规使用监测中的应用及研究进展  

Application and research progress of metabonomics technology in monitoring violatory use of veterinary drugs

作　　者：郭旭茜 韩雪[1] 李研东 蔡英华[3] 于雷[3] 康牧旭 艾连峰 GUO Xuqian;HAN Xue;LI Yandong;CAI Yinghua;YU Lei;KANG Muxu;AI Lianfeng(School of Bioscience and Bioengineering,Hebei University of Science and Technology,Shijiazhuang,Hebei 050018,China;Hebei Institute of Veterinary Drugs Control,Shijiazhuang,Hebei 050051,China;China Animal Disease Prevention and Control Center,Beijing 102618,China;Xingtai Institute of Food and Drug Inspection,Xingtai,Hebei 054000,China;Hebei Entry-Exit Inspection and Quarantine Technical Center,Shijiazhuang,Hebei 050073,China)

机构地区：[1]河北科技大学生物科学与工程学院,河北石家庄050018 [2]河北省兽药监察所,河北石家庄050051 [3]中国动物疫病预防控制中心,北京102618 [4]邢台市食品药品检验所,河北邢台054000 [5]河北出入境检验检疫局检验检疫技术中心,河北石家庄050073

出　　处：《河北科技大学学报》2019年第4期359-365,共7页Journal of Hebei University of Science and Technology

基　　金：河北省重点研发计划项目(19227124D);邢台市科技计划项目(2018ZC232)

摘　　要：兽药残留分析检测技术的开发与创新是动物源性食品安全监测的重要研究内容。作为系统生物学的重要组成部分,代谢组学研究及其检测技术在动物源性食品安全领域发挥了不可或缺的作用。基于当前代谢组学技术在兽药残留检测领域的研究成果,阐述了代谢组学的概念、原理与分类;以"瘦肉精"和类固醇两类违禁兽药残留检测为例,简述了液相色谱串联质谱法(liquid chromatography-tandem mass spectrometry,LC-MS)、气相色谱串联质谱法(gas chromatographytandem mass spectrometry,GC-MS)等代谢组学常用检测技术对动物血液、尿液等生物样本进行残留分析检测的应用现状,分析了非靶向代谢组学与靶向代谢组学存在的精确度差与通量低等问题,以及代谢组学技术对大型仪器设备与专业数据库过度依赖的局限性;进一步提出了代谢组学技术在兽药残留检测的应用中应根据样品特点及检测目的,将多技术平台(液相色谱-电喷雾飞行质谱、超高效液相色谱-四级杆飞行时间质谱)、分析平台(主成分分析、偏最小二乘判别分析)与多组学技术(基因组学、转录组学、蛋白组学)联合使用以提高代谢组学技术在兽药残留检测领域的适用性与应用范围,为兽药残留分析检测提供新的研究方向。The development and innovation of veterinary drug residue analysis and detection technology is an important research content of animal-derived food safety monitoring.As an important part of systems biology,metabolomics research and its detection technology also play an indispensable role in the field of animal-derived food safety.Based on the current research results of metabolomics technology in the field of veterinary drug residue detection,the conceptual,principles and classification of metabolomics are illustrated.Taking the detection of two types of veterinary drug residues,such as clenbuterol and steroids,the current application status of the commonly used detection techniques of metabolomics contains liquid chromatography-tandem mass spectrometry(LC-MS)or gas chromatography-tandem mass spectrometry(GC-MS)on the detection of animal's blood and urine samples.The problems of untargeted metabolomics and targeted metabolomics with low precision and throughput are analyzed.The limitations of metabolomics technology over-reliance on large instrumentation and professional databases have also been offered.Further,metabolomics technology is proposed,which should be based on sample characteristics and detection purposes in the application of veterinary drug residue detection.The multi-technology platform(liquid chromatography electrospray time-of-flight mass spectrometry,ultra high performance liquid chromatography quadrupole time-of-flight mass spectrometry),multi-analysis platform(principal component analysis,partial least squares discrimination analysis)and multi-omics(genomics,transcriptomics,and proteomics)technology are used together to improve the applicability and applicational range of metabolomics technology in the field of veterinary drug residue detection.It provides new research directions for veterinary drug residue analysis and testing technology.

关 键 词：食品检验学 代谢组学 兽药残留 食品安全 检测 

分 类 号：Q591.1[生物学—生物化学]