植物乳杆菌YW11杀菌型发酵乳调节肠道菌群结构  

Regulation of Intestinal Flora Structure by Pasteurized Fermented Milk with Lactobacillus plantarum YW11

作　　者：郝晓娜 罗天淇 张健[1] 赵笑[1] 余志坚 曹永强 杨贞耐[1] HAO Xiaona;LUO Tianqi;ZHANG Jian;ZHAO Xiao;YU Zhijian;CAO Yongqiang;YANG Zhennai(Beijing Advanced Innovation Center for Food Nutrition and Human Health,Beijing Engineering and Technology Research Center ofFood Additives,Beijing Technology and Business University,Beijing 100048,China;Dongjun (Yucheng) Dairy Co. Ltd.,Dezhou 253000,China)

机构地区：[1]北京食品营养与人类健康高精尖创新中心,北京市食品添加剂工程技术研究中心,北京工商大学,北京100048 [2]东君乳业(禹城)有限公司,山东德州253000

出　　处：《食品科学》2019年第15期169-176,共8页Food Science

基　　金：国家自然科学基金面上项目(31571857)

摘　　要：为了研究用益生性植物乳杆菌YW11制备的巴氏杀菌发酵乳对人体肠道菌群结构的调节作用,通过构建体外模拟人体消化模型,采用高通量16S rRNA测序技术研究菌株YW11制备的巴氏杀菌发酵乳对肠道菌群多样性和组成结构的影响。结果显示,该杀菌型发酵乳能增加肠道微生态中菌群的丰富度和多样性。在门水平,YW11巴氏杀菌发酵乳能增加厚壁菌门的丰度,减少变形菌门的丰度;在属水平,YW11巴氏杀菌发酵乳能提高高丰度菌群的丰度,增加肠道中45.71%菌群的丰度。另外,YW11巴氏杀菌发酵乳能增加产丁酸的毛螺旋菌属、罗斯氏菌、瘤胃球菌属的丰度,增加乳酸菌属和双歧杆菌的丰度。同时,该杀菌发酵乳还能增加与缓解炎症性肠病(inflammatory bowel disease,IBD)相关的拟杆菌属、粪栖杆菌、考拉菌属、柯林斯菌属的丰度,减少与诱发IBD炎症相关丹毒丝菌属、假单胞菌属、志贺埃希氏菌的丰度,表明其对人体肠道菌群的影响趋于有益的方向。因此,YW11巴氏杀菌发酵乳具有调节人体肠道菌群的作用。实验结果可为益生性发酵乳的开发提供理论依据。In order to explore the influence of pasteurized fermented milk with probiotic Lactobacillus plantarum YW11 on the human intestinal flora structure, an in vitro simulated human digestion model was established and high-throughput16 S rRNA sequencing was applied to analyze the diversity and composition of the intestinal flora. Results indicated that the pasteurized fermented milk could increase the richness and diversity of intestinal microbial species. At the phylum level,it increased the abundance of Firmicutes and reduced the abundance of Proteobacteria. At the genus level, it increased the abundance of highly enriched bacteria and the abundance of 45.71% of the intestinal flora. In addition, the fermented milk increased the abundance of butyric acid-producing bacteria, such as Lachnospiraceae, Roseburia, Ruminococcus,and the abundance of Lactobacillus and Bifidobacterium. At the same time, the fermented milk also increased the bacteria implicated in relieving inflammatory bowel disease(IBD), such as Bacteroides, Faecalibacterium, Phascolarctobacterium and Collinsella, and reduced those related to the development of IBD, such as Erysipelotrichaceae, Pseudomonas and Escherichia-Shigella, indicating the beneficial effect of the pasteurized fermented milk on the human intestinal flora.Therefore, this fermented milk can function to regulate the human intestinal flora. These experimental results can provide a theoretical basis for the development of probiotic-fermented milk.

关 键 词：植物乳杆菌YW11 巴氏杀菌发酵乳 炎症性肠病 肠道菌群 

分 类 号：TS252.5[轻工技术与工程—农产品加工及贮藏工程]