一株高产Monacolin K紫红曲霉菌株筛选、鉴定及发酵条件优化  

Screening,identification and fermentation condition optimization of a high Monacolin K producing Monascus purpureus

作　　者：赵娜 张红星 谢远红 金君华 贾宇 ZHAO Na;ZHANG Hong-xing;XIE Yuan-hong;JIN Jun-hua;JIA Yu(Beijing University of Agricultue,Beijing,Food Science and Engineering College,Beijing Key Laboratory of Detection and Control of Spoilage Organisms and Pesticide Residues in Agricultural Products,Beijing Laboratory of Food Quality and Safety,Key Technology Development ofMicroecological Preparations Beijing Engineering Laboratory,Beijing102206 ,China)

机构地区：[1]北京农学院食品科学与工程学院农产品有害微生物及农残安全检测与控制北京市重点实验室食品质量与安全北京实验室微生态制剂关键技术开发北京市工程实验室

出　　处：《天然产物研究与开发》2019年第8期1326-1331,1325,共7页Natural Product Research and Development

基　　金：北京农学院研究生改革与发展项目(2018YJS031)

摘　　要：为从天然发酵红曲米中分离的30株红曲霉菌株中筛选高产MonacolinK的菌株,并对其产MonacolinK的发酵条件进行优化。实验采用高效液相色谱法(HPLC)筛选到9株具有产MonacolinK能力的红曲霉菌株,其中以编号ZX26的菌株产MonacolinK能力最高,发酵液中Monacolin K产量达到107.6mg/L,并且产MonacolinK能力具有良好的稳定性。微生物形态学结合ITS基因同源性分析结果表明,编号ZX26菌株为紫红曲霉。进一步采用单因素试验和正交试验法优化紫红曲霉ZX26产MonacolinK的发酵条件,结果表明在培养基组分为葡萄糖70g/L,牛肉膏15g/L,NaNO32g/L,MgSO4·7H2O0.5g/L,KH2PO41.5g/L时,其最优发酵条件为:发酵初始pH4.0,接种量为7%,培养温度30℃,发酵10天,在此条件下,紫红曲霉ZX26发酵液中MonacolinK产量达到271.36mg/L,相对于培养条件优化前MonacolinK产量提高152.19%,经验证此培养条件下MonacolinK产量最佳。The purpose of this study was to screen the high Monacolin K-producing strains from30 strains of Monascus Strain separated from natural fermented red yeast rice,and further to optimize the fermentation conditions of Monacolin K.The results showed that 9 strains of Monascus were found to produce Monacolin K by high performance liquid chromatography (HPLC) analysis.The strain No.ZX26 was the strongest strain in producing Monacolin K,and the yield of Monacolin K in the fermentation broth reached up to107.6 mg/L.The ITS gene homology analysis showed that Monascus Strain ZX26 was Monascus purpureus. The single factor test and orthogonal test method were used to optimize the fermentation conditions of Monacolin K produced by Monascus purpureus ZX26 further.The results showed that,when the composition of the medium was glucose 70 g/L,beef extract15 g/L,NaNO32 g/L,MgSO 4·7H2O 0.5 g/L and KH2PO 41.5 g/L,the optimal fermentation conditions including the initial pH of 4.0,culture temperature of30 ℃,inoculum size of 7% and fermentation time of10 days were determined.On the optimal fermentation conditions,the yield of Monacolin K reached up to271.36mg/L,which increased by152.19% compared with the production of Monacolin K before optimizing fermentation conditions,It was proved that the Monacolin K yield was the best under this cultivation condition.

关 键 词：红曲霉 MONACOLINK 高效液相色谱 正交试验 

分 类 号：R939.9[医药卫生—生药学]