Isolation, characterization and effect of plant-growth-promoting rhizobacteria on pine seedlings(Pinus pseudostrobus Lindl.)  

作　　者：Cristina Heredia-Acuna Juan J.Almaraz-Suarez Ramon Arteaga-Garibay Ronald Ferrera-Cerrato Deisy Y.Pineda-Mendoza 

机构地区：[1]Area de Microbiologia,Postgrado de Edafologia,Colegio de Postgraduados,Carretera Mexico-Texcoco km 36.5,CP 56230 Montecillo,Estado de Mexico,Mexico [2]Laboratorio de Recursos Geneticos Microbianos,Centro Nacional de Recursos Geneticos,INIFAP,Boulevard de la Biodiversidad No.400,CP 47600 Tepatitlan de Morelos,Jalisco,Mexico

出　　处：《Journal of Forestry Research》2019年第5期1727-1734,共8页林业研究（英文版）

基　　金：supported by the project ‘‘Impact of Climatic Change and Agricultural Activity on the Emission of Greenhouse Gases and on the Microbial Resources of the Sierra Nevada,Mexico’’[‘‘Impacto del Cambio Climáticos y actividad agrícola en la emisión de gases de efecto invernadero y en los recursos microbianos de la Sierra Nevada,México’’] No.213059;funded by the National Council of Science and Technology [Consejo Nacional de Ciencia y Tecnología(CONACy T)]

摘　　要：In this study,10 bacterial strains were isolated from the rhizosphere of coniferous trees on Mount Tla′loc in Mexico.The strains were characterized by their capacity to produce auxins,solubilize phosphates and stimulate mycelial growth of the ectomycorrhizal fungus Suillus sp.All isolates were identified at the molecular level.Moreover,an experiment was established to evaluate the response of Pinus pseudostrobus seedlings to inoculation with the rhizobacteria strains.The isolated strains belonged to the species Cupriavidus basilensis,Rhodococcus qingshengii,R.erythropolis,Pseudomonas spp.,P.gessardii,Stenotrophomonas rhizophila and Cohnella sp.All of the strains produced auxins;the best producer was R.erythropolis CPT9(76.4 lg mL^-1).P.gessardii CPT6 solubilized phosphate at a significant level(443 lg mL^-1).The strain S.rhizophila CPT8 significantly increased the radial growth of the ectomycorrhizal fungus Suillus sp.by 18.8%.Five strains increased the dry mass of the shoots;R.qingshengii CPT4 and R.erythropolis CPT9 increased growth the most,by more than 20%.Inoculation with plant-growth-promoting rhizobacteria can be a very useful practice in a forest nursery to produce healthy,vigorous plants.In this study,10 bacterial strains were isolated from the rhizosphere of coniferous trees on Mount Tlaloc in Mexico.The strains were characterized by their capacity to produce auxins,solubilize phosphates and stimulate mycelial growth of the ectomycorrhizal fungus Suillus sp.All isolates were identified at the molecular level.Moreover,an experiment was established to evaluate the response of Pinus pseudostrobus seedlings to inoculation with the rhizobacteria strains.The isolated strains belonged to the species Cupriavidus basilensis,Rhodococcus qingshengii,R.erythropolis,Pseudo,monas spp.,P.gessardii,Stenotropho,monas rhizophila and Cohnella sp.All of the strains produced auxins;the best producer was R.erythropolis CPT9（76.4 μg mL-1）.P.gessardii CPT6 solubilized phosphate at a significant level(443 μg mL-1).The strain S.rhizophila CPT8 significantly increased the radial growth of the ectomycorrhizal fungus Suillus sp.by18.8%.Five strains increased the dry mass of the shoots;R.qingshengii CPT4 and R.erythropolis CPT9 increased growth the most,by more than 20%.Inoculation with plant-growth-promoting rhizobacteria can be a very useful practice in a forest nursery to produce healthy,vigorous plants.

关 键 词：BIOFERTILIZERS Forest species RHIZOBACTERIA INDOLE acetic acid PHOSPHATE SOLUBILIZATION 

分 类 号：S[农业科学]