不同温度对弓鲤鱼与弓鲇鱼乳酸盐代谢网络通量的影响  

Effects of Different Temperatures on Lactate Metabolic Network Flux in Bow-Bound Carp and Bow-Bound Catfish

作　　者：袁帅 庞广昌 YUAN Shuai;PANG Guangchang(Tianjin Key Laboratory of Food Biotechnology,College of Biotechnology and Food Science,Tianjin University of Commerce,Tianjin 300134,China)

机构地区：[1]天津商业大学生物技术与食品科学学院天津市食品生物技术重点实验室

出　　处：《食品科学》2019年第17期53-58,共6页Food Science

基　　金：国家自然科学基金自由申请项目（31671857）

摘　　要：本研究以鲤鱼和鲇鱼为实验材料,利用弓鱼技术研究不同温度对鱼类中心代谢网络通量的影响以及弓鱼在最适温度下离水存活的时间,并探究低温诱导鲤鱼与鲇鱼进入休眠以及实现活鱼离水贮运的可行性。将弓鲤鱼和弓鲇鱼分别放置于0、4、6、10、15℃不同温度下(每个温度采用梯度降温),测定不同温度下弓鲤鱼与弓鲇鱼血清中糖酵解途径、三羧酸循环和磷酸戊糖途径的代谢通量。结果显示,鲤鱼在6℃、鲇鱼在4℃时的中心代谢途径中代表合成代谢的戊糖途径通量最高;而代表分解代谢和呼吸强度的乳酸盐代谢通量最低,因此判定6℃和4℃分别为鲤鱼和鲇鱼的最优贮运温度,且可分别作为诱导鲤鱼与鲇鱼进入休眠的最适温度。进而在最适温度下进行存活实验,结果显示,在6℃时鲤鱼离水存活时间平均可达48.93 h,最长存活时间为77 h;在4℃时鲇鱼离水存活时间平均可达38.14 h,最长存活时间为70 h。以上结果表明,活鱼的离水贮藏运输是完全可行的,同时本实验为诱导鱼类进入冬眠或休眠提供新的方法和理论基础。In this experiment,we studied the effects of different temperatures on the central metabolic network flux in bow-bound carp and catfish and determined their survival time without water at the optimum temperature determined in this study.Besides,we also explored the feasibility of low temperature dormancy and waterless live fish transportation for carp and cat fish.At temperatures of 0,4,6,10 and 15℃(gradual cooling to each of the temperatures),the metabolic fluxes in the glycolytic pathway,the tricarboxylic acid cycle and the pentose phosphate pathway were determined in the serum of fish.The results showed that the pentose pathway flux,representing anabolism,was the highest among the central metabolic pathways for bow-bounded carp at 6℃and for bow-bounded cat fish at 4℃,while the lactate metabolism flux,representing catabolism and respiratory intensity,was the lowest which provides evidence that 6℃is the optimal storage temperature and dormancy temperature for carp,and 4℃for catfish.Furthermore,the survival experiment showed that the survival time of carp was 48.93 h on average and 77 h at best,while the survival time of cat fish was 38.14 h on average and 70 h at best.The above results indicated the feasibility of waterless storage and transportation for live bow-bound fish.This study provides a new method and theoretical basis for inducing hibernation and sleep in fish.

关 键 词：鲤鱼 鲇鱼 弓鱼 冬眠 乳酸盐 代谢通量 

分 类 号：TS201.1[轻工技术与工程—食品科学]