苜蓿皂苷和酵母培养物对肉羊体外瘤胃发酵特性和甲烷产量的影响  

Effects of Alfalfa Saponin and Yeast Cultures on Rumen Fermentation Characteristics and Methane Production of Mutton Sheep in Vitro

作　　者：徐晨晨[1] 郭娉婷[1] 刘策 曲扬华 丁兰兰[1] 张昊[2] 罗海玲[1] XU Chenchen;GUO Pingting;LIU Ce;QU Yanghua;DING Lanlan;ZHANG Hao;LUO Hailing(State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China)

机构地区：[1]中国农业大学动物科技学院,动物营养学国家重点实验室,北京100193 [2]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《动物营养学报》2019年第9期4226-4234,共9页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家肉羊产业技术体系资助项目(CARS-38);农业部公益性行业专项(201303091)

摘　　要：本试验旨在利用体外培养法研究苜蓿皂苷和酵母培养物对肉羊体外瘤胃发酵特性、甲烷产量和营养物质降解率的影响。试验选用3只健康、体重(67.0±1.3) kg、安装永久性瘤胃瘘管的36月龄杜寒杂交羯羊作为试验动物,用于采集瘤胃液。试验采用3×3水平设计,在发酵底物中添加不同水平(0、22、44 mg/g DM)的苜蓿皂苷和不同水平(0、44、88 mg/g DM)的酵母培养物,通过体外产气技术进行48 h体外模拟瘤胃发酵试验。结果表明:1)苜蓿皂苷和酵母培养物的互作效应对累计产气量、产气动力学参数、甲烷产量和瘤胃发酵液参数均影响显著(P<0.05)。2)随苜蓿皂苷添加水平的增加,累计产气量和产气动力学参数均线性升高(P <0. 05),而瘤胃液pH及丙酸、丁酸含量均线性降低(P<0.05),甲烷产量呈现二次降低的变化趋势(P<0.05)。3)随酵母培养物添加水平的增加,产气速率、最大产气量1/2时的产气速率和瘤胃液总挥发性脂肪酸含量均线性升高(P<0.05),甲烷产量呈现二次降低的变化趋势(P<0.05),瘤胃液pH呈现出线性和二次降低的变化趋势(P <0. 05)。4)苜蓿皂苷和酵母培养物及二者的互作效应对体外营养物质降解率均无显著影响(P>0.05)。由此可见,苜蓿皂苷和酵母培养物的互作效应对瘤胃发酵参数和甲烷产量的作用效果更优,且以44 mg/g DM的苜蓿皂苷和88 mg/g DM的酵母培养物组合时最佳。The purpose of this study was to investigate the effects of alfalfa saponin and yeast cultures on rumen fermentation characteristics, methane production and nutrient degradation rate of mutton sheep in vitro. Three healthy Dorper×thin-tailed Han crossbred wethers with an average body weight of (67.0±1.3) kg fitted with permanent ruminal fistulas were used for rumen fluid collection. A 3×3 factorial arrangement design was adopted in the experiment, and supplemented with different levels (0, 22 and 44 mg/g DM) of alfalfa saponin and different levels (0, 44 and 88 mg/g DM) of yeast cultures in the fermentation substrate, and carried out by in vitro gas production technique for 48 h in vitro simulated rumen fermentation experiment. The results showed as follows:1) the interaction effect of alfalfa saponin and yeast cultures had significant effects on cumulative gas production, gas production kinetics parameters, methane production and rumen fermentation fluid parameters in vitro (P<0.05). 2) With the alfalfa saponin supplemental level increasing, the cumulative gas production and gas production kinetics parameters were linearly increased (P<0.05), while the pH and contents of propionic acid and butyric acid in rumen fluid were linearly decreased (P<0.05), and the methane production showed a quadratic decrease trend (P<0.05). 3) With the yeast cultures supplemental level increasing, the gas production rate, gas production rate when gas production was 1/2 of the maximum and content of total volatile fatty acids in rumen fluid were linearly increased (P<0.05), the methane production showed a quadratic decrease trend (P<0.05), and the pH in rumen fluid showed linearly and quadratic decrease trend (P<0.05). 4) The alfalfa saponin and yeast cultures and their interaction effect had no significant effects on nutrient degradation rates in vitro (P>0.05). In conclusion, the interaction effect of alfalfa saponin and yeast cultures has better effects on rumen fermentation parameters and methane production, and the best combi

关 键 词：苜蓿皂苷 酵母培养物 瘤胃 体外发酵 甲烷产量 

分 类 号：S826[农业科学—畜牧学]