基于新一代高通量测序技术分析拮抗酵母Cryptoccocus laurentii诱导处理樱桃番茄果实后转录组学的变化  

Transcriptome Analysis of Cherry Tomato Fruit Treated with Cryptoccocus laurentii Using High-Throughput Next-Generation Sequencing Technology

作　　者：唐琼 郑晓冬[1] TANG Qiong;ZHENG Xiaodong(College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China)

机构地区：[1]浙江大学生物系统工程与食品科学学院

出　　处：《食品科学》2019年第18期55-62,共8页Food Science

基　　金：国家自然科学基金面上项目（31571897）

摘　　要：罗伦隐球酵母(Cryptococcus laurentii)作为生物激发子,能显著诱导采后樱桃番茄对灰葡萄孢(Botrytiscinerea)的抗性响应,但其防治机制尚未完全明确。本实验以樱桃番茄为材料,利用新一代高通量测序技术Illumina HiSeqTM4000对罗伦隐球酵母处理及水对照处理的番茄果肉组织进行转录组测序,采用生物信息学方法对差异基因进行功能注释和通路分析,并利用实时荧光定量聚合酶链式反应(real time-quantitative polymerase chainreaction,RT-qPCR)技术对随机选取的差异表达基因进行验证。结果表明,樱桃番茄果实经过罗伦隐球酵母诱导处理48 h后,共有3 141个基因差异表达,上、下调表达基因分别为1 689个和1 452个,这些差异表达基因涉及多个代谢路径,揭示罗伦隐球酵母对番茄的诱导作用是一个多代谢路径参与的调控过程。此外,RT-qPCR验证结果与转录组分析结果趋势一致。As a biological elicitor, Cryptococcus laurentii can significantly induce resistance to Botrytis cinerea in cherry tomato but the underlying mechanism is still ambiguous. High-throughput sequencing on an Illumina HiSeqTM 4000 platform was used for transcriptome sequencing of cherry tomato fruit treated with C. laurentii or sterile water, and bioinformatic methods were used for gene function prediction. Then the expression levels of some defense-related genes were monitored by real time-quantitative polymerase chain reaction(RT-qPCR). By transcriptome sequencing, it was found that there were a total of 3 141 differentially expressed genes in tomato fruit treated with C. laurentii for 48 h, 1 689 of which were up-regulated while 1 452 were down-regulated. These differentially expressed genes were involved in multiple metabolic pathways, which implied that the inductive effect of C. laurentii on cherry tomato fruit was a collaborative process. Moreover, the RT-qPCR results were consistent with the transcriptome analysis.

关 键 词：罗伦隐球酵母 樱桃番茄 转录组学 实时荧光定量聚合酶链式反应 

分 类 号：Q945.8[农业科学—植物病理学]